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Literature summary for 3.2.1.81 extracted from

  • Minegishi, H.; Shimane, Y.; Echigo, A.; Ohta, Y.; Hatada, Y.; Kamekura, M.; Maruyama, T.; Usami, R.
    Thermophilic and halophilic beta-agarase from a halophilic archaeon Halococcus sp. 197A (2013), Extremophiles, 17, 931-939.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
salt requirement for stability Halococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
KCl no activity in the presence of 0–0.3 M salt. The enzyme shows a salt requirement for activity, being active from 0.3 M NaCl, with maximal activity at 3.5 M NaCl. KCl supports similar activities as NaCl up to 3.5 M, and LiCl up to 2.5 M. The monovalent salts can not be substituted by 3.5 M divalent cations, CaCl2 or MgCl2 Halococcus sp.
LiCl no activity in the presence of 0–0.3 M salt. The enzyme shows a salt requirement for activity, being active from 0.3 M NaCl, with maximal activity at 3.5 M NaCl. KCl supports similar activities as NaCl up to 3.5 M, and LiCl up to 2.5 M. The monovalent salts can not be substituted by 3.5 M divalent cations, CaCl2 or MgCl2 Halococcus sp.
NaCl no activity in the presence of 0-0.3 M salt. The enzyme shows a salt requirement for activity, being active from 0.3 M NaCl, with maximal activity at 3.5 M NaCl. KCl supports similar activities as NaCl up to 3.5 M, and LiCl up to 2.5 M. The monovalent salts can not be substituted by 3.5 M divalent cations, CaCl2 or MgCl2 Halococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
1 * 55000, SDS-PAGE Halococcus sp.
56000
-
gel filtration Halococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agar + H2O Halococcus sp. the enzyme degrades agarose into neoagarooligosaccharides. The neoagarooligosaccharides have various special biological activities, such as inhibition of bacterial growth, slowing down of starch degradation thereby reducing the calorific value of food, and providing anticancer, antivirus, and anti-oxidation activities neoagarobiose + neoagarotetraose + neoagarohexaose
-
?
agar + H2O Halococcus sp. 197A the enzyme degrades agarose into neoagarooligosaccharides. The neoagarooligosaccharides have various special biological activities, such as inhibition of bacterial growth, slowing down of starch degradation thereby reducing the calorific value of food, and providing anticancer, antivirus, and anti-oxidation activities neoagarobiose + neoagarotetraose + neoagarohexaose
-
?

Organism

Organism UniProt Comment Textmining
Halococcus sp.
-
-
-
Halococcus sp. 197A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Halococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agar + H2O the enzyme degrades agarose into neoagarooligosaccharides. The neoagarooligosaccharides have various special biological activities, such as inhibition of bacterial growth, slowing down of starch degradation thereby reducing the calorific value of food, and providing anticancer, antivirus, and anti-oxidation activities Halococcus sp. neoagarobiose + neoagarotetraose + neoagarohexaose
-
?
agar + H2O the enzyme releases degradation products in the order of neoagarohexose, neoagarotetraose and small quantity of neoagarobiose Halococcus sp. neoagarobiose + neoagarotetraose + neoagarohexaose
-
?
agar + H2O the enzyme degrades agarose into neoagarooligosaccharides. The neoagarooligosaccharides have various special biological activities, such as inhibition of bacterial growth, slowing down of starch degradation thereby reducing the calorific value of food, and providing anticancer, antivirus, and anti-oxidation activities Halococcus sp. 197A neoagarobiose + neoagarotetraose + neoagarohexaose
-
?
agar + H2O the enzyme releases degradation products in the order of neoagarohexose, neoagarotetraose and small quantity of neoagarobiose Halococcus sp. 197A neoagarobiose + neoagarotetraose + neoagarohexaose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Halococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Halococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 85 45°C: about 50% of maximal activity, 85°C: about 55% of maximal activity Halococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65 80 the enzyme retains approximately 90% of the initial activity after incubation for 1 h at 65-80°C Halococcus sp.
95
-
the enzyme retains 50% activity after 1 h at 95°C Halococcus sp.
100
-
in the presence of 10 mM CaCl2, approximately 17% remaining activity is detected after 30 min Halococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Halococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 35% of maximal activity, about 40% of maximal activity Halococcus sp.