General Stability | Organism |
---|---|
salt requirement for stability | Halococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | no activity in the presence of 00.3 M salt. The enzyme shows a salt requirement for activity, being active from 0.3 M NaCl, with maximal activity at 3.5 M NaCl. KCl supports similar activities as NaCl up to 3.5 M, and LiCl up to 2.5 M. The monovalent salts can not be substituted by 3.5 M divalent cations, CaCl2 or MgCl2 | Halococcus sp. | |
LiCl | no activity in the presence of 00.3 M salt. The enzyme shows a salt requirement for activity, being active from 0.3 M NaCl, with maximal activity at 3.5 M NaCl. KCl supports similar activities as NaCl up to 3.5 M, and LiCl up to 2.5 M. The monovalent salts can not be substituted by 3.5 M divalent cations, CaCl2 or MgCl2 | Halococcus sp. | |
NaCl | no activity in the presence of 0-0.3 M salt. The enzyme shows a salt requirement for activity, being active from 0.3 M NaCl, with maximal activity at 3.5 M NaCl. KCl supports similar activities as NaCl up to 3.5 M, and LiCl up to 2.5 M. The monovalent salts can not be substituted by 3.5 M divalent cations, CaCl2 or MgCl2 | Halococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
1 * 55000, SDS-PAGE | Halococcus sp. |
56000 | - |
gel filtration | Halococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | Halococcus sp. | the enzyme degrades agarose into neoagarooligosaccharides. The neoagarooligosaccharides have various special biological activities, such as inhibition of bacterial growth, slowing down of starch degradation thereby reducing the calorific value of food, and providing anticancer, antivirus, and anti-oxidation activities | neoagarobiose + neoagarotetraose + neoagarohexaose | - |
? | |
agar + H2O | Halococcus sp. 197A | the enzyme degrades agarose into neoagarooligosaccharides. The neoagarooligosaccharides have various special biological activities, such as inhibition of bacterial growth, slowing down of starch degradation thereby reducing the calorific value of food, and providing anticancer, antivirus, and anti-oxidation activities | neoagarobiose + neoagarotetraose + neoagarohexaose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halococcus sp. | - |
- |
- |
Halococcus sp. 197A | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Halococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | the enzyme degrades agarose into neoagarooligosaccharides. The neoagarooligosaccharides have various special biological activities, such as inhibition of bacterial growth, slowing down of starch degradation thereby reducing the calorific value of food, and providing anticancer, antivirus, and anti-oxidation activities | Halococcus sp. | neoagarobiose + neoagarotetraose + neoagarohexaose | - |
? | |
agar + H2O | the enzyme releases degradation products in the order of neoagarohexose, neoagarotetraose and small quantity of neoagarobiose | Halococcus sp. | neoagarobiose + neoagarotetraose + neoagarohexaose | - |
? | |
agar + H2O | the enzyme degrades agarose into neoagarooligosaccharides. The neoagarooligosaccharides have various special biological activities, such as inhibition of bacterial growth, slowing down of starch degradation thereby reducing the calorific value of food, and providing anticancer, antivirus, and anti-oxidation activities | Halococcus sp. 197A | neoagarobiose + neoagarotetraose + neoagarohexaose | - |
? | |
agar + H2O | the enzyme releases degradation products in the order of neoagarohexose, neoagarotetraose and small quantity of neoagarobiose | Halococcus sp. 197A | neoagarobiose + neoagarotetraose + neoagarohexaose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Halococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Halococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 85 | 45°C: about 50% of maximal activity, 85°C: about 55% of maximal activity | Halococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 80 | the enzyme retains approximately 90% of the initial activity after incubation for 1 h at 65-80°C | Halococcus sp. |
95 | - |
the enzyme retains 50% activity after 1 h at 95°C | Halococcus sp. |
100 | - |
in the presence of 10 mM CaCl2, approximately 17% remaining activity is detected after 30 min | Halococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Halococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 35% of maximal activity, about 40% of maximal activity | Halococcus sp. |