Any feedback?
Literature summary for 3.2.1.81 extracted from
- A unique beta-agarase, AgaA, from a marine bacterium, Vibrio sp. strain PO-303 (2007), Appl. Environ. Microbiol., 74, 1248-1255.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ligated into a pET22b(+) vector and expressed in Escherichia coli BL21(DE3) competent cells | Vibrio sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | partially inhibits the activity | Vibrio sp. |  |
| AlCl3 | completely inhibits the activity | Vibrio sp. | |
| CuCl2 | completely inhibits the activity | Vibrio sp. | |
| CuSO4 | completely inhibits the activity | Vibrio sp. | |
| FeCl3 | partially inhibits the activity | Vibrio sp. | |
| HgCl2 | completely inhibits the activity | Vibrio sp. | |
| MnCl2 | partially inhibits the activity | Vibrio sp. | |
| additional information | NaCl, KCl, MgCl2, and BaCl2 negligibly affect the enzymatic activity | Vibrio sp. | |
| Pb(CH3COO)2 | completely inhibits the activity | Vibrio sp. | |
| SDS | 0.1% SDS completely inhibits the activity | Vibrio sp. | |
| ZnCl2 | completely inhibits the activity | Vibrio sp. | |
| ZnSO4 | completely inhibits the activity | Vibrio sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | slightly enhances enzymatic activity | Vibrio sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 106100 | - |
sequence analysis | Vibrio sp. |
| 120000 | - |
1 * 120000, SDS-PAGE | Vibrio sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 18.8fold and with a final yield of 16.8% by anion exchange and hydrophobic chromatographies | Vibrio sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.872 | - |
cell lysate | Vibrio sp. |
| 16.4 | - |
18.8fold purified enzyme | Vibrio sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agar + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? | |
| agarose + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? | |
| additional information | does not act on neoagarotetraose and neoagarobiose and kappa-carrageenan | Vibrio sp. | ? | - |
? | |
| neoagarohexaose + H2O | - |
Vibrio sp. | ? | - |
? | |
| neoagarooctaose + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose + neoagarobiose | - |
? | |
| porphyran + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 120000, SDS-PAGE | Vibrio sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgaA | - |
Vibrio sp. |
| beta-agarase | - |
Vibrio sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Vibrio sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
stable under 37°C after a incubation of 10 min | Vibrio sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Vibrio sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 11 | after preincubated at various pHs at 25°C for 12 h | Vibrio sp. |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Vibrio sp. | - |
- |
4.14 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
