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Literature summary for 3.2.1.81 extracted from
- Purification and characterization of a novel beta-agarase, AgaA34, from Agarivorans albus YKW-34 (2008), Appl. Microbiol. Biotechnol., 78, 265-273.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 30% increase in activity | Agarivorans albus |  |
| dithiothreitol | 30% increase in activity | Agarivorans albus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | strong | Agarivorans albus | |
| Cu2+ | strong | Agarivorans albus | |
| Fe3+ | strong | Agarivorans albus | |
| Mn2+ | strong | Agarivorans albus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | ratio of Km/kcat value for hydrolysis of neoagarohexaose and neoagarotetraose is 4040 and 810 per s and M, respectively | Agarivorans albus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Agarivorans albus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ions required for activity | Agarivorans albus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Agarivorans albus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agarivorans albus | - |
isolated from gut of a turban shell | - |
| Agarivorans albus YKW-34 | - |
isolated from gut of a turban shell | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from culture supernatant | Agarivorans albus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | - |
Agarivorans albus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.5 | - |
40°C, pH 8.0 | Agarivorans albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Agarivorans albus | neoagarobiose + neoagarotetraose | about 75% neoagarobiose plus 25% neoagarotetraose | ? | |
| agarose + H2O | - |
Agarivorans albus YKW-34 | neoagarobiose + neoagarotetraose | about 75% neoagarobiose plus 25% neoagarotetraose | ? | |
| additional information | no substrate: alginate, carboxymethyl cellulose, fucoidan, kappa-carrageenan, lambda-carrageenan | Agarivorans albus | ? | - |
? | |
| additional information | no substrate: alginate, carboxymethyl cellulose, fucoidan, kappa-carrageenan, lambda-carrageenan | Agarivorans albus YKW-34 | ? | - |
? | |
| neoagarohexaose + H2O | - |
Agarivorans albus | neoagarobiose | - |
? | |
| neoagarohexaose + H2O | - |
Agarivorans albus YKW-34 | neoagarobiose | - |
? | |
| neoagarotetraose + H2O | - |
Agarivorans albus | neoagarobiose | - |
? | |
| neoagarotetraose + H2O | - |
Agarivorans albus YKW-34 | neoagarobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, SDS-PAGE | Agarivorans albus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Agarivorans albus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
90% of maximum activity | Agarivorans albus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, 95% residual activity. Inactivation above 50°C | Agarivorans albus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Agarivorans albus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | more than 80% of maximum activity | Agarivorans albus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | - |
Agarivorans albus |
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