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Literature summary for 3.2.1.81 extracted from

  • Leon, O.; Quintana, L.; Peruzzo, P.; Slebe, J.C.
    Purification and properties of an extracellular agarase from Alteromonas sp. strain C-1 (1992), Appl. Environ. Microbiol., 58, 4060-4063.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.81

Inhibitors

Inhibitors Comment Organism Structure
KCl 50% inhibition at 0.1 M Alteromonas sp.
NaCl 50% inhibition at 0.1 M Alteromonas sp.
NH4Cl 50% inhibition at 0.1 M Alteromonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 neoagarohexaose
-
 Alteromonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Alteromonas sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 gel filtration Alteromonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Alteromonas sp.
-
 neoagarotetraose
-
 ir

Organism

Organism UniProt Comment Textmining
Alteromonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alteromonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
234
-
-
 Alteromonas sp.

Storage Stability

Storage Stability Organism
4°C, Tris-HCl with 0.4 M NaCl, pH 7.5, more than 6 months Alteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Alteromonas sp. neoagarotetraose
-
 ir

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 inhibition above 30°C, after 20 min 35°C 50% of activity Alteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Alteromonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
 NaCl
-
 Alteromonas sp.
0.1
-
 KCl
-
 Alteromonas sp.
0.1
-
 NH4Cl
-
 Alteromonas sp.