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Literature summary for 3.2.1.81 extracted from

  • Ha, J.C.; Kim, G.T.; Kim, S.K.; Oh, J.H.; Kong, I.S.
    beta-Agarase from Pseudomonas sp. W7: purification and recombinant enzyme from Escherichia coli and the effects of salt on its activity (1997), Biotechnol. Appl. Biochem., 26, 1-6.
No PubMed abstract available
Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vibrio sp.
expression in Escherichia coli Pseudoalteromonas atlantica

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 0.9 M Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
-
 Pseudoalteromonas atlantica
59000
-
 SDS-PAGE Pseudomonas sp.
105000
-
-
 Vibrio sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Pseudomonas sp.
-
 neoagarotetraose
-
 ir
agarose + H2O Vibrio sp.
-
 neoagarotetraose
-
 ir
agarose + H2O Pseudoalteromonas atlantica
-
 neoagarotetraose
-
 ir

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas atlantica
-
-
-
Pseudomonas sp.
-
-
-
Vibrio sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43.3
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Pseudomonas sp. neoagarotetraose
-
 ir
agarose + H2O
-
 Vibrio sp. neoagarotetraose
-
 ir
agarose + H2O
-
 Pseudoalteromonas atlantica neoagarotetraose
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 40
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Pseudomonas sp.