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Literature summary for 3.2.1.80 extracted from
- A thermostable exo-beta-fructosidase immobilised through rational design (2014), Food Chem., 145, 826-831.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the C-terminally His-tagged extracellular enzyme in Pichia pastoris strain PpBfrA(4X), secretion to the culture medium, fed-batch fermentation | Thermotoga maritima |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilisation of the purified recombinant N-glycosylated enzyme designed to incorporate the optimal amount of protein/activity per surface area of aldehyde-activated glyoxyl-resin at pH 8.0-10.0, method overview | Thermotoga maritima |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, deglycosylated recombinant enzyme, SDS-PAGE | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Thermotoga maritima | the enzyme shows invertase activity releasing fructose and glucose from sucrose, EC 3.2.1.26 | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | removal of N-linked oligosaccharides by endoglycoside H treatment | Thermotoga maritima |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant extracellular His-tagged enzyme from Pichia pastoris strain PpBfrA(4X) culture supernatant by nickel affinity chromatography | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme shows invertase activity releasing fructose and glucose from sucrose, EC 3.2.1.26 | Thermotoga maritima | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, deglycosylated recombinant enzyme, SDS-PAGE | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BfrA | - |
Thermotoga maritima |
| exo-beta-fructosidase | - |
Thermotoga maritima |
| invertase | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | the recombinant immonbilized enzyme retains 88%, 77%, and 57% of its initial activity after preincubation for 10 days at temperatures of 60°C, 70°C, and 80°C, respectively. The soluble enzyme is inactivated at a higher rate, overview | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Thermotoga maritima |
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Release 2023.1 (January 2023)
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