Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.80 extracted from

  • Arrizon, J.; Morel, S.; Gschaedler, A.; Monsan, P.
    Purification and substrate specificities of a fructanase from Kluyveromyces marxianus isolated from the fermentation process of Mezcal (2011), Biores. Technol., 102, 3298-3303.
    View publication on PubMed
Search for more literature for 3.2.1.80

Inhibitors

Inhibitors Comment Organism Structure
Ag+ more than 50% of residual enzymatic activity at 20 mM Kluyveromyces marxianus
Ba2+ more than 50% of residual enzymatic activity at 20 mM Kluyveromyces marxianus
Cu2+ less than 50% of residual enzymatic activity at 20 mM Kluyveromyces marxianus
EDTA
-
 Kluyveromyces marxianus
Fe2+ more than 50% of residual enzymatic activity at 20 mM Kluyveromyces marxianus
Fe3+ more than 50% of residual enzymatic activity at 20 mM Kluyveromyces marxianus
Hg2+ less than 50% of residual enzymatic activity at 20 mM Kluyveromyces marxianus
Mn2+ less than 50% of residual enzymatic activity at 20 mM Kluyveromyces marxianus
Zn2+ less than 50% of residual enzymatic activity at 20 mM Kluyveromyces marxianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 levan purified enzyme, at pH 5.0 and 50°C Kluyveromyces marxianus
7.16
-
 inulin purified enzyme, at pH 5.0 and 55°C Kluyveromyces marxianus
12.9
-
 Agave tequilana fructan purified enzyme, at pH 5.0 and 50°C Kluyveromyces marxianus
27.8
-
 Actilight purified enzyme, at pH 5.0 and 55°C Kluyveromyces marxianus
29.1
-
 sucrose purified enzyme, at pH 5.0 and 55°C Kluyveromyces marxianus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10% increase of activity at 20 mM Ca2+ Kluyveromyces marxianus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
 2 * 125000, SDS-PAGE Kluyveromyces marxianus
250000
-
 gel filtration Kluyveromyces marxianus

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Kluyveromyces marxianus

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration and Mono Q Sepharose column chromatography Kluyveromyces marxianus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.02
-
 crude extract, at pH 5.0 and 50°C Kluyveromyces marxianus
49.5
-
 after 12.3fold purification, at pH 5.0 and 50°C Kluyveromyces marxianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Actilight + H2O highest activity, Actilight is a commercial mixture composed of 4.8% (w/v) of free sugars (sucrose, glucose and fructose), 35.1% w/v of 1-kestose, 53% w/v of 1-nystose and 7.1% w/v of 1-fructofuranosyl-nystose Kluyveromyces marxianus ?
-
 ?
Agave tequilana fructan + H2O
-
 Kluyveromyces marxianus ?
-
 ?
inulin + 5 H2O
-
 Kluyveromyces marxianus 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
levan + H2O
-
 Kluyveromyces marxianus D-fructose + ?
-
 ?
sucrose + H2O highest activity Kluyveromyces marxianus beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 125000, SDS-PAGE Kluyveromyces marxianus

Synonyms

Synonyms Comment Organism
Fructanase
-
 Kluyveromyces marxianus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 the optimal temperature for levan and Agave tequilana fructan hydrolysis is 50°C Kluyveromyces marxianus
55
-
 the optimal temperature for inulin, Actilight and sucrose hydrolysis is 55°C Kluyveromyces marxianus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 the enzyme shows 100%, 84.2%, 98.6%, 92.9% and 100% residual activity after 72 h at 40°C when using levan, inulin, Agave tequilana ructan, Actilight and sucrose as substrates, respectively Kluyveromyces marxianus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 inulin purified enzyme, at pH 5.0 and 55°C Kluyveromyces marxianus
0.33
-
 levan purified enzyme, at pH 5.0 and 50°C Kluyveromyces marxianus
0.9
-
 Agave tequilana fructan purified enzyme, at pH 5.0 and 50°C Kluyveromyces marxianus
1.09
-
 Actilight purified enzyme, at pH 5.0 and 55°C Kluyveromyces marxianus
1.27
-
 sucrose purified enzyme, at pH 5.0 and 55°C Kluyveromyces marxianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 6.5 for Agave tequilana fructan as substrate, the enzymatic activity is constant from pH 4.0 to 6.5 Kluyveromyces marxianus
4.5 5 using levan as substrate Kluyveromyces marxianus
5
-
 using inulin, Actilight and sucrose as substrates Kluyveromyces marxianus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
 inulin purified enzyme, at pH 5.0 and 55°C Kluyveromyces marxianus
0.18
-
 levan purified enzyme, at pH 5.0 and 50°C Kluyveromyces marxianus
0.9
-
 Agave tequilana fructan purified enzyme, at pH 5.0 and 50°C Kluyveromyces marxianus
1.09
-
 Actilight purified enzyme, at pH 5.0 and 55°C Kluyveromyces marxianus
1.27
-
 sucrose purified enzyme, at pH 5.0 and 55°C Kluyveromyces marxianus