Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.80 extracted from

  • Le Roy, K.; Lammens, W.; Verhaest, M.; De Coninck, B.; Rabijns, A.; Van Laere, A.; Van den Ende, W.
    Unraveling the difference between invertases and fructan exohydrolases: a single amino acid (Asp-239) substitution transforms Arabidopsis cell wall invertase1 into a fructan 1-exohydrolase (2007), Plant Physiol., 145, 616-625.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.80

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information a D239A mutant of cell wall invertase 1 acts as a 1-FEH, preferentially degrading 1-kestose Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
sucrose
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 1-kestose in 50 mM sodium acetate buffer, pH 5.0, at 30°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q67XZ3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O
-
 Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
1-FEH
-
 Arabidopsis thaliana
1-KEH
-
 Arabidopsis thaliana
1-kestose exohydrolase
-
 Arabidopsis thaliana
AtcwINV1 D239A mutant
-
 Arabidopsis thaliana
fructan 1-exohydrolase
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
 1-kestose in 50 mM sodium acetate buffer, pH 5.0, at 30°C Arabidopsis thaliana