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Literature summary for 3.2.1.80 extracted from

  • Le Roy, K.; Verhaest, M.; Rabijns, A.; Clerens, S.; Van Laere, A.; Van den Ende, W.
    N-glycosylation affects substrate specificity of chicory fructan 1-exohydrolase: evidence for the presence of an inulin binding cleft (2007), New Phytol., 176, 317-324.
    View publication on PubMed
Search for more literature for 3.2.1.80

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Cichorium intybus
expression in Pichia pastoris Cichorium intybus

Protein Variants

Protein Variants Comment Organism
P294N mutant with an introduced N-glycosylation site near the inulin-binding cleft, shows highly decreased activity against higher DP inulin. The introduction of a glycosyl chain most probably blocks the cleft and prevents inulin binding and degradation Cichorium intybus
P294N shows highly decreased activity against inulin Cichorium intybus

Inhibitors

Inhibitors Comment Organism Structure
additional information glycosylation of 1-FEH IIa affected overall enzyme activity Cichorium intybus

Organism

Organism UniProt Comment Textmining
Cichorium intybus
-
-
-
Cichorium intybus Q93X60 precursor
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycosylation of 1-FEH IIa affects overall enzyme activity Cichorium intybus

Purification (Commentary)

Purification (Comment) Organism
-
 Cichorium intybus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O
-
 Cichorium intybus beta D-fructofuranose + sucrose
-
 ?
1-kestose + H2O
-
 Cichorium intybus beta-D-fructose + sucrose
-
 ?
fructan + H2O
-
 Cichorium intybus beta-D-fructose + ?
-
 ?
inulin + 5 H2O
-
 Cichorium intybus 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Synonyms

Synonyms Comment Organism
1-FEH IIa
-
 Cichorium intybus
fructan 1-exohydrolase
-
 Cichorium intybus