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Literature summary for 3.2.1.80 extracted from
- Partial purification and characterization of exoinulinase from Kluyveromyces marxianus YS-1 for preparation of high-fructose syrup (2007), J. Microbiol. Biotechnol., 17, 733-738.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme preparation is used to hydrolyze pure inulin and raw inulin from Asparagus racemosus for the preparation of a high-fructose syrup | Kluyveromyces marxianus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | potent inhibitor below 1 mM | Kluyveromyces marxianus |  |
| CuSO4 | 1 mM, 47% inhibition | Kluyveromyces marxianus | |
| EDTA | 1 mM, 50% inhibition | Kluyveromyces marxianus | |
| Fe2(SO4)3 | 1 mM, 38% inhibition | Kluyveromyces marxianus | |
| Hg2+ | potent inhibitor below 1mM | Kluyveromyces marxianus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
raffinose | pH 5.5, 50°C | Kluyveromyces marxianus | |
| 3.4 | - |
inulin | pH 5.5, 50°C | Kluyveromyces marxianus | |
| 25.3 | - |
sucrose | pH 5.5, 50°C | Kluyveromyces marxianus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Kluyveromyces marxianus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | maximal increase in activity: 1.2fold | Kluyveromyces marxianus | |
| Mn2+ | maximal increase in activity: 2.4fold | Kluyveromyces marxianus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces marxianus | - |
- |
- |
| Kluyveromyces marxianus Ys-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Kluyveromyces marxianus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 413 | - |
- |
Kluyveromyces marxianus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | - |
Kluyveromyces marxianus | D-glucose + D-fructose + ? | - |
? | |
| inulin + H2O | - |
Kluyveromyces marxianus Ys-1 | D-glucose + D-fructose + ? | - |
? | |
| raffinose + H2O | hydrolyzed at 69% of the activity with inulin | Kluyveromyces marxianus | beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside | - |
? | |
| raffinose + H2O | hydrolyzed at 69% of the activity with inulin | Kluyveromyces marxianus Ys-1 | beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside | - |
? | |
| sucrose + H2O | hydrolyzed at 177.6% of the activity with inulin | Kluyveromyces marxianus | D-fructose + D-glucose | - |
? | |
| sucrose + H2O | hydrolyzed at 177.6% of the activity with inulin | Kluyveromyces marxianus Ys-1 | D-fructose + D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exoinulinase | - |
Kluyveromyces marxianus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Kluyveromyces marxianus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
3 h, stable | Kluyveromyces marxianus |
| 60 | - |
3 h, 50% loss of activity | Kluyveromyces marxianus |
| 70 | - |
3 h, 85% loss of activity | Kluyveromyces marxianus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Kluyveromyces marxianus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | 50°C, 3 h, stable | Kluyveromyces marxianus |
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