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Literature summary for 3.2.1.80 extracted from

  • Nagem, R.A.; Rojas, A.L.; Golubev, A.M.; Korneeva, O.S.; Eneyskaya, E.V.; Kulminskaya, A.A.; Neustroev, K.N.; Polikarpov, I.
    Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognition (2004), J. Mol. Biol., 344, 471-480.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme-fructose-complex, 1.87 A resolution, single isomorphous replacement, 20% fructose solution Aspergillus awamori
free enzyme, 1.5 A resolution, single isomorphous replacement, orthorhombic crystals, 0.5 M sodium iodide and flash-cooling to -173°C in stream of cold nitrogen Aspergillus awamori

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus awamori
-
-

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
EI
-
Aspergillus awamori EI
-
EI
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Aspergillus awamori

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus awamori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
Aspergillus awamori beta-D-fructose + ?
-
?
inulin + H2O
-
Aspergillus awamori EI beta-D-fructose + ?
-
?
levan + H2O
-
Aspergillus awamori beta-D-fructose + ?
-
?
levan + H2O
-
Aspergillus awamori EI beta-D-fructose + ?
-
?
sucrose + H2O
-
Aspergillus awamori beta-D-fructose + D-glucose
-
?
sucrose + H2O
-
Aspergillus awamori EI beta-D-fructose + D-glucose
-
?