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Literature summary for 3.2.1.80 extracted from

  • Van Riet, L.; Nagaraj, V.; Van den Ende, W.; Clerens, S.; Wiemken, A.; van Laere, A.
    Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.) (2006), J. Exp. Bot., 57, 213-223.
    View publication on PubMed
Search for more literature for 3.2.1.80

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Pichia pastoris Triticum aestivum

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by sucrose Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 SDS-PAGE Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
 cv. Pajero
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, ConA affinity-, ion exchange-chromatography, and gel filtration Triticum aestivum

Reaction

Reaction Comment Organism Reaction ID
1,1,1-kestose + H2O = beta-D-fructose + sucrose exclusive hydrolysis of beta-2-6 bonds in fructan-producing plants Triticum aestivum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.65
-
 native protein, pH 5.0 Triticum aestivum
26.6
-
 protein expressed in Pichia pastoris, pH 5.0 Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-kestose + H2O
-
 Triticum aestivum beta-D-fructose + sucrose
-
 ?
levan + H2O
-
 Triticum aestivum beta-D-fructose + ?
-
 ?
levanbiose + H2O
-
 Triticum aestivum beta-D-fructose
-
 ?
additional information no activity towards sucrose, 1-kestose, nystose, inulin Triticum aestivum ?
-
 ?
phlein + H2O
-
 Triticum aestivum beta-D-fructose + ?
-
 ?

Synonyms

Synonyms Comment Organism
6-FEH
-
 Triticum aestivum