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Literature summary for 3.2.1.80 extracted from
- Purification and some important characters of extracellular inulinase of Alternaria alternata (Fr.) Keissler (2002), Indian J. Exp. Biol., 40, 1393-1398.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | microbial production of high fructose syrup, alcohol, acetone and butanol | Alternaria alternata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 30 min at 30ÂșC, 10 mM, 70% inhibition; 30 min at 30ÂșC, 1 mM, 17% inhibition; 30 min at 30ÂșC, 5 mM, 39% inhibition | Alternaria alternata |  |
| Cu2+ | 30 min at 30ÂșC, 10 mM, 100% inhibition; 30 min at 30ÂșC, 1 mM, 23% inhibition; 30 min at 30ÂșC, 5 mM, 87% inhibition | Alternaria alternata | |
| EDTA | 30 min at 30ÂșC, 10 mM, 10% inhibition; 30 min at 30ÂșC, 5 mM, 9% inhibition | Alternaria alternata | |
| Fe3+ | 30 min at 30ÂșC, 10 mM, 86% inhibition; 30 min at 30ÂșC, 1 mM, 19% inhibition; 30 min at 30ÂșC, 5 mM, 60% inhibition | Alternaria alternata | |
| Hg2+ | 30 min at 30ÂșC, 10 mM, 85% inhibition; 30 min at 30ÂșC, 1 mM, 26% inhibition; 30 min at 30ÂșC, 5 mM, 57% inhibition | Alternaria alternata | |
| iodoacetate | 30 min at 30ÂșC, 10 mM, 87% inhibition; 30 min at 30ÂșC, 1 mM, 38% inhibition; 30 min at 30ÂșC, 5 mM, 57% inhibition | Alternaria alternata | |
| Mn2+ | 30 min at 30ÂșC, 10 mM, 20% inhibition | Alternaria alternata | |
| Sodium arsenate | 30 min at 30ÂșC, 10 mM, 50% inhibition; 30 min at 30ÂșC, 1 mM, 20% inhibition; 30 min at 30ÂșC, 5 mM, 38% inhibition | Alternaria alternata | |
| sodium arsenite | 30 min at 30ÂșC, 10 mM, 58% inhibition; 30 min at 30ÂșC, 1 mM, 28% inhibition; 30 min at 30ÂșC, 5 mM, 41% inhibition | Alternaria alternata | |
| Zn2+ | 30 min at 30ÂșC, 10 mM, 66% inhibition; 30 min at 30ÂșC, 1 mM, 22% inhibition; 30 min at 30ÂșC, 5 mM, 39% inhibition | Alternaria alternata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 66 | - |
inulin | at 55ÂșC, pH 4.6 | Alternaria alternata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Alternaria alternata | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 30 min at 30ÂșC, 1 mM, 118% of the relative activity without metal ions | Alternaria alternata | |
| Ba2+ | 30 min at 30ÂșC, 10 mM, 140% of the relative activity without metal ions | Alternaria alternata | |
| Ba2+ | 30 min at 30ÂșC, 5 mM, 132% of the relative activity without metal ions | Alternaria alternata | |
| Ca2+ | 30 min at 30ÂșC, 1 mM, 118% of the relative activity without metal ions | Alternaria alternata | |
| Ca2+ | 30 min at 30ÂșC, 5 mM, 108% of the relative activity without metal ions | Alternaria alternata | |
| Mg2+ | 30 min at 30ÂșC, 1 mM, 110% of the relative activity without metal ions | Alternaria alternata | |
| Mn2+ | 30 min at 30ÂșC, 1 mM, 109% of the relative activity without metal ions | Alternaria alternata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | - |
gel filtration | Alternaria alternata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | Alternaria alternata | - |
oligofructosides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alternaria alternata | - |
(Fr.) Keissler | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | - |
Alternaria alternata |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ammonium sulfate precipitation, Sephadex column and DEAE-cellulose column, 62fold | Alternaria alternata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Alternaria alternata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [”mol/min/mg] | Specific Activity Maximum [”mol/min/mg] | Comment | Organism |
|---|---|---|---|
| 80700 | - |
- |
Alternaria alternata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| at either -15ÂșC or at 4ÂșC, 200 mM acetate buffer, pH 4.6, 2 weeks, 50% loss of activity | Alternaria alternata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | - |
Alternaria alternata | oligofructosides | - |
? | |
| raffinose + H2O | - |
Alternaria alternata | beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranose | - |
? | |
| sucrose + H2O | - |
Alternaria alternata | beta-D-fructose + alpha-D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exoinulinase | - |
Alternaria alternata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
pH 4.6 | Alternaria alternata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
after 50ÂșC there is a gradual loss in activity, pH 4.6 | Alternaria alternata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
at 55ÂșC, enzyme retains only 90% of its activity | Alternaria alternata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | at 55ÂșC | Alternaria alternata |
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