Cloned (Comment) | Organism |
---|---|
gene xynA, DNA and amino acid sequence determination and analysis, recombinant expression in Yarrowia lipolytica under control of the strong constitutive TEF promoter with the preproLIP2 secretion signal using plasmid JMP62_TEF_Ura3Ex, the enzyme is secreted. Recombinant expression in Pichia pastoris strain GS115 results in only low enzyme activity | Penicillium citrinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km of the recombinant enzyme for birchwood xylan is 5.2 mg/ml, pH 5.0, 55°C | Penicillium citrinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium citrinum | Q2PGY1 | - |
- |
Penicillium citrinum FERM P-15944 | Q2PGY1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant extrecellular enzyme is highly glycosylated | Penicillium citrinum |
Purification (Comment) | Organism |
---|---|
recombinant extracellular enzyme 185fold, the enzyme is deglycosylated by EndoH, followed by ultrafiltration, and anion exchange chromatography | Penicillium citrinum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
695.2 | - |
purified recombinant deglycosylated extracellular enzyme, pH 5.0, 55°C, substrate birchwood xylan | Penicillium citrinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azo-xylan + H2O | - |
Penicillium citrinum | ? | - |
? | |
azo-xylan + H2O | - |
Penicillium citrinum FERM P-15944 | ? | - |
? | |
birchwood xylan + H2O | - |
Penicillium citrinum | xylobiose + xylotriose + ? | - |
? | |
birchwood xylan + H2O | - |
Penicillium citrinum FERM P-15944 | xylobiose + xylotriose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000, recombinant extracellular deglycosylated enzyme, SDS-PAGE, x * 60000, above, recombinant extracellular glycosylated enzyme, SDS-PAGE | Penicillium citrinum |
Synonyms | Comment | Organism |
---|---|---|
xylanase A | - |
Penicillium citrinum |
XynA | - |
Penicillium citrinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme | Penicillium citrinum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | recombinant enzyme, over 50% of maximal activity within this range, profile overview | Penicillium citrinum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant extracellular deglycosylated enzyme, 6 h, over 80% activity remains | Penicillium citrinum |
45 | - |
purified recombinant extracellular deglycosylated enzyme, 70% activity remains after 30 min, 17% after 3 h, and 6% after 5 h | Penicillium citrinum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
245 | - |
birchwood xylan | recombinant enzyme, pH 5.0, 55°C | Penicillium citrinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
recombinant enzyme | Penicillium citrinum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6.5 | recombinant enzyme, over 50% of maximal activity within this range, profile overview | Penicillium citrinum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | purified recombinant extracellular deglycosylated enzyme, 24 h, over 80% activity remains | Penicillium citrinum |