Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | slightly activating | Aspergillus flavus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 27% inhibition at 1 mM | Aspergillus flavus | |
additional information | poor inhibition by DTT and EDTA. Various proteases have poor effects on the xylanase AfXynA, overview | Aspergillus flavus | |
Zn2+ | 16% inhibition at 1 mM | Aspergillus flavus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Aspergillus flavus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no or poor effects by Mg2+, Ba2+, Fe2+, Fe3+, Ca2+, K+, Na+, and Cu2+ at 1 mM | Aspergillus flavus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
corncob xylan + H2O | Aspergillus flavus | - |
xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | Aspergillus flavus SRRC 167 | - |
xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | Aspergillus flavus ATCC 200026 | - |
xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | Aspergillus flavus FGSC A1120 | - |
xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | Aspergillus flavus NRRL 3357 | - |
xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | Aspergillus flavus JCM 12722 | - |
xylobiose + xylotriose + xylopentose + xylohexaose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | B8NKE9 | - |
- |
Aspergillus flavus ATCC 200026 | B8NKE9 | - |
- |
Aspergillus flavus FGSC A1120 | B8NKE9 | - |
- |
Aspergillus flavus JCM 12722 | B8NKE9 | - |
- |
Aspergillus flavus NRRL 3357 | B8NKE9 | - |
- |
Aspergillus flavus SRRC 167 | B8NKE9 | - |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme from culture supernatant 3.7fold by ammonium sulfate fractionation and gel filtration | Aspergillus flavus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus flavus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
838.2 | - |
purified native extracellular enzyme XynA, pH 7.5, 55°C, substrate beechwood xylan | Aspergillus flavus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beechwood xylan + H2O | - |
Aspergillus flavus | xylobiose + xylotriose | - |
? | |
beechwood xylan + H2O | - |
Aspergillus flavus SRRC 167 | xylobiose + xylotriose | - |
? | |
beechwood xylan + H2O | - |
Aspergillus flavus ATCC 200026 | xylobiose + xylotriose | - |
? | |
beechwood xylan + H2O | - |
Aspergillus flavus FGSC A1120 | xylobiose + xylotriose | - |
? | |
beechwood xylan + H2O | - |
Aspergillus flavus NRRL 3357 | xylobiose + xylotriose | - |
? | |
beechwood xylan + H2O | - |
Aspergillus flavus JCM 12722 | xylobiose + xylotriose | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus | xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus SRRC 167 | xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus ATCC 200026 | xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus FGSC A1120 | xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus NRRL 3357 | xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus JCM 12722 | xylobiose + xylotriose + xylopentose + xylohexaose | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus | xylobiose + xylotriose + ? | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus SRRC 167 | xylobiose + xylotriose + ? | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus ATCC 200026 | xylobiose + xylotriose + ? | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus FGSC A1120 | xylobiose + xylotriose + ? | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus NRRL 3357 | xylobiose + xylotriose + ? | - |
? | |
corncob xylan + H2O | - |
Aspergillus flavus JCM 12722 | xylobiose + xylotriose + ? | - |
? | |
additional information | the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside | Aspergillus flavus | ? | - |
? | |
additional information | the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside | Aspergillus flavus SRRC 167 | ? | - |
? | |
additional information | the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside | Aspergillus flavus ATCC 200026 | ? | - |
? | |
additional information | the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside | Aspergillus flavus FGSC A1120 | ? | - |
? | |
additional information | the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside | Aspergillus flavus NRRL 3357 | ? | - |
? | |
additional information | the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside | Aspergillus flavus JCM 12722 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20200, extracellular enzyme, SDS-PAGE | Aspergillus flavus |
Synonyms | Comment | Organism |
---|---|---|
AfXynA | - |
Aspergillus flavus |
beta-1, 4-endoxylanase | - |
Aspergillus flavus |
xlnA | - |
Aspergillus flavus |
XynA | - |
Aspergillus flavus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Aspergillus flavus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified extracellular enzyme, stable up to | Aspergillus flavus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Aspergillus flavus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 10.5 | purified extracellular enzyme, stable at | Aspergillus flavus |