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Literature summary for 3.2.1.8 extracted from

  • Chen, Z.; Zaky, A.A.; Liu, Y.; Chen, Y.; Liu, L.; Li, S.; Jia, Y.
    Purification and characterization of a new xylanase with excellent stability from Aspergillus flavus and its application in hydrolyzing pretreated corncobs (2019), Protein Expr. Purif., 154, 91-97 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
SDS slightly activating Aspergillus flavus

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 27% inhibition at 1 mM Aspergillus flavus
additional information poor inhibition by DTT and EDTA. Various proteases have poor effects on the xylanase AfXynA, overview Aspergillus flavus
Zn2+ 16% inhibition at 1 mM Aspergillus flavus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Aspergillus flavus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no or poor effects by Mg2+, Ba2+, Fe2+, Fe3+, Ca2+, K+, Na+, and Cu2+ at 1 mM Aspergillus flavus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
corncob xylan + H2O Aspergillus flavus
-
xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O Aspergillus flavus SRRC 167
-
xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O Aspergillus flavus ATCC 200026
-
xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O Aspergillus flavus FGSC A1120
-
xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O Aspergillus flavus NRRL 3357
-
xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O Aspergillus flavus JCM 12722
-
xylobiose + xylotriose + xylopentose + xylohexaose
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus flavus B8NKE9
-
-
Aspergillus flavus ATCC 200026 B8NKE9
-
-
Aspergillus flavus FGSC A1120 B8NKE9
-
-
Aspergillus flavus JCM 12722 B8NKE9
-
-
Aspergillus flavus NRRL 3357 B8NKE9
-
-
Aspergillus flavus SRRC 167 B8NKE9
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from culture supernatant 3.7fold by ammonium sulfate fractionation and gel filtration Aspergillus flavus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus flavus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
838.2
-
purified native extracellular enzyme XynA, pH 7.5, 55°C, substrate beechwood xylan Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
Aspergillus flavus xylobiose + xylotriose
-
?
beechwood xylan + H2O
-
Aspergillus flavus SRRC 167 xylobiose + xylotriose
-
?
beechwood xylan + H2O
-
Aspergillus flavus ATCC 200026 xylobiose + xylotriose
-
?
beechwood xylan + H2O
-
Aspergillus flavus FGSC A1120 xylobiose + xylotriose
-
?
beechwood xylan + H2O
-
Aspergillus flavus NRRL 3357 xylobiose + xylotriose
-
?
beechwood xylan + H2O
-
Aspergillus flavus JCM 12722 xylobiose + xylotriose
-
?
corncob xylan + H2O
-
Aspergillus flavus xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O
-
Aspergillus flavus SRRC 167 xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O
-
Aspergillus flavus ATCC 200026 xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O
-
Aspergillus flavus FGSC A1120 xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O
-
Aspergillus flavus NRRL 3357 xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O
-
Aspergillus flavus JCM 12722 xylobiose + xylotriose + xylopentose + xylohexaose
-
?
corncob xylan + H2O
-
Aspergillus flavus xylobiose + xylotriose + ?
-
?
corncob xylan + H2O
-
Aspergillus flavus SRRC 167 xylobiose + xylotriose + ?
-
?
corncob xylan + H2O
-
Aspergillus flavus ATCC 200026 xylobiose + xylotriose + ?
-
?
corncob xylan + H2O
-
Aspergillus flavus FGSC A1120 xylobiose + xylotriose + ?
-
?
corncob xylan + H2O
-
Aspergillus flavus NRRL 3357 xylobiose + xylotriose + ?
-
?
corncob xylan + H2O
-
Aspergillus flavus JCM 12722 xylobiose + xylotriose + ?
-
?
additional information the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside Aspergillus flavus ?
-
?
additional information the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside Aspergillus flavus SRRC 167 ?
-
?
additional information the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside Aspergillus flavus ATCC 200026 ?
-
?
additional information the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside Aspergillus flavus FGSC A1120 ?
-
?
additional information the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside Aspergillus flavus NRRL 3357 ?
-
?
additional information the extracellular xylanase has an endohydrolytic mode of action and hydrolyzes xylotriose to xylobiose through transglycosylation. It can efficiently degrade xylan to mainly yield xylobiose, xylotriose, xylopentose and xylohexaose. In addition, enzyme AfXynA is effective in hydrolyzing pretreated corncobs. No activity with carboxymethyl cellulose, barley glucan, and debranched arabinan or the 4-nitrophenyl glycosides such as 4-nitrophenyl-beta-xylopyranoside Aspergillus flavus JCM 12722 ?
-
?

Subunits

Subunits Comment Organism
? x * 20200, extracellular enzyme, SDS-PAGE Aspergillus flavus

Synonyms

Synonyms Comment Organism
AfXynA
-
Aspergillus flavus
beta-1, 4-endoxylanase
-
Aspergillus flavus
xlnA
-
Aspergillus flavus
XynA
-
Aspergillus flavus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Aspergillus flavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified extracellular enzyme, stable up to Aspergillus flavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Aspergillus flavus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 10.5 purified extracellular enzyme, stable at Aspergillus flavus