Application | Comment | Organism |
---|---|---|
additional information | the enzyme can be used for various biotechnological applications such as for prebiotic xylooligosaccharides and bioethanol production from pretreated agrowaste biomass | Pseudopedobacter saltans |
Cloned (Comment) | Organism |
---|---|
gene Pedsa_2704, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain TOP10 | Pseudopedobacter saltans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 2 mM | Pseudopedobacter saltans | |
Ba2+ | 42% inhibition at 2 mM | Pseudopedobacter saltans | |
Co2+ | 39% inhibition at 2 mM | Pseudopedobacter saltans | |
Cu2+ | 85% inhibition at 2 mM | Pseudopedobacter saltans | |
EDTA | 36% inhibition at 2 mM | Pseudopedobacter saltans | |
guanidine hydrochloride | 84% inhibition at 2 M | Pseudopedobacter saltans | |
Hg2+ | complete inhibition at 2 mM | Pseudopedobacter saltans | |
Li+ | 54% inhibition at 2 mM | Pseudopedobacter saltans | |
Ni2+ | 48% inhibition at 2 mM | Pseudopedobacter saltans | |
Pb2+ | 65% inhibition at 2 mM | Pseudopedobacter saltans | |
SDS | 55% inhibition at 0.25% w/v | Pseudopedobacter saltans | |
Urea | 80% inhibition at 0.5 M | Pseudopedobacter saltans | |
Zn2+ | 51% inhibition at 2 mM | Pseudopedobacter saltans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten and Lineweaver-Burk kinetics, Km for beechwood xylan is 6.2 mg/ml for birchwood 3.9 mg/ml, and for xylan MW 2000-30000 5.1 mg/ml | Pseudopedobacter saltans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates by 29% at 2 mM | Pseudopedobacter saltans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudopedobacter saltans | F0S6Y3 | i.e. Pedobacter saltans | - |
Pseudopedobacter saltans ATCC 51119 | F0S6Y3 | i.e. Pedobacter saltans | - |
Pseudopedobacter saltans DSM 12145 | F0S6Y3 | i.e. Pedobacter saltans | - |
Pseudopedobacter saltans JCM 21818 | F0S6Y3 | i.e. Pedobacter saltans | - |
Pseudopedobacter saltans LMG 10337 | F0S6Y3 | i.e. Pedobacter saltans | - |
Pseudopedobacter saltans NBRC 100064 | F0S6Y3 | i.e. Pedobacter saltans | - |
Pseudopedobacter saltans NCIMB 13643 | F0S6Y3 | i.e. Pedobacter saltans | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Pseudopedobacter saltans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8 | - |
purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate oat spelt xylan | Pseudopedobacter saltans |
12.7 | - |
purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate insoluble wheat arabinoxylan | Pseudopedobacter saltans |
18.7 | - |
purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate insoluble rye arabinoxylan | Pseudopedobacter saltans |
22.3 | - |
purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate soluble wheat arabinoxylan | Pseudopedobacter saltans |
25.4 | - |
purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate soluble rye arabinoxylan | Pseudopedobacter saltans |
37.3 | - |
purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate birchwood xylan | Pseudopedobacter saltans |
47 | - |
purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate 4-O methyl-glucuronoxylan | Pseudopedobacter saltans |
57.1 | - |
purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate xylan with MW of 20000-30000 | Pseudopedobacter saltans |
59.7 | - |
purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate beechwood xylan | Pseudopedobacter saltans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O methyl-glucuronoxylan + H2O | moderate activity | Pseudopedobacter saltans | ? | - |
? | |
beechwood xylan + H2O | best substrate | Pseudopedobacter saltans | xylose + xylobiose + xylotetraose | - |
? | |
birchwood xylan + H2O | moderate activity | Pseudopedobacter saltans | ? | - |
? | |
birchwood xylan + H2O | moderate activity | Pseudopedobacter saltans NBRC 100064 | ? | - |
? | |
birchwood xylan + H2O | moderate activity | Pseudopedobacter saltans JCM 21818 | ? | - |
? | |
birchwood xylan + H2O | moderate activity | Pseudopedobacter saltans NCIMB 13643 | ? | - |
? | |
birchwood xylan + H2O | moderate activity | Pseudopedobacter saltans ATCC 51119 | ? | - |
? | |
birchwood xylan + H2O | moderate activity | Pseudopedobacter saltans LMG 10337 | ? | - |
? | |
birchwood xylan + H2O | moderate activity | Pseudopedobacter saltans DSM 12145 | ? | - |
? | |
additional information | enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview | Pseudopedobacter saltans | ? | - |
? | |
additional information | enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview | Pseudopedobacter saltans NBRC 100064 | ? | - |
? | |
additional information | enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview | Pseudopedobacter saltans JCM 21818 | ? | - |
? | |
additional information | enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview | Pseudopedobacter saltans NCIMB 13643 | ? | - |
? | |
additional information | enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview | Pseudopedobacter saltans ATCC 51119 | ? | - |
? | |
additional information | enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview | Pseudopedobacter saltans LMG 10337 | ? | - |
? | |
additional information | enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview | Pseudopedobacter saltans DSM 12145 | ? | - |
? | |
oat spelt xylan + H2O | low activity | Pseudopedobacter saltans | ? | - |
? | |
oat spelt xylan + H2O | low activity | Pseudopedobacter saltans NBRC 100064 | ? | - |
? | |
oat spelt xylan + H2O | low activity | Pseudopedobacter saltans JCM 21818 | ? | - |
? | |
oat spelt xylan + H2O | low activity | Pseudopedobacter saltans NCIMB 13643 | ? | - |
? | |
oat spelt xylan + H2O | low activity | Pseudopedobacter saltans ATCC 51119 | ? | - |
? | |
oat spelt xylan + H2O | low activity | Pseudopedobacter saltans LMG 10337 | ? | - |
? | |
oat spelt xylan + H2O | low activity | Pseudopedobacter saltans DSM 12145 | ? | - |
? | |
rye arabinoxylan + H2O | soluble and insoluble, the latter is less active, moderate to lower activity | Pseudopedobacter saltans | ? | - |
? | |
wheat arabinoxylan + H2O | soluble and insoluble, the latter is less active, moderate to lower activity | Pseudopedobacter saltans | ? | - |
? | |
wheat arabinoxylan + H2O | soluble and insoluble, the latter is less active, moderate to lower activity | Pseudopedobacter saltans NBRC 100064 | ? | - |
? | |
wheat arabinoxylan + H2O | soluble and insoluble, the latter is less active, moderate to lower activity | Pseudopedobacter saltans JCM 21818 | ? | - |
? | |
wheat arabinoxylan + H2O | soluble and insoluble, the latter is less active, moderate to lower activity | Pseudopedobacter saltans NCIMB 13643 | ? | - |
? | |
wheat arabinoxylan + H2O | soluble and insoluble, the latter is less active, moderate to lower activity | Pseudopedobacter saltans ATCC 51119 | ? | - |
? | |
wheat arabinoxylan + H2O | soluble and insoluble, the latter is less active, moderate to lower activity | Pseudopedobacter saltans LMG 10337 | ? | - |
? | |
wheat arabinoxylan + H2O | soluble and insoluble, the latter is less active, moderate to lower activity | Pseudopedobacter saltans DSM 12145 | ? | - |
? | |
xylan + H2O | molecular weight of 20000-30000, high activity | Pseudopedobacter saltans | ? | - |
? | |
xylan + H2O | molecular weight of 20000-30000, high activity | Pseudopedobacter saltans NBRC 100064 | ? | - |
? | |
xylan + H2O | molecular weight of 20000-30000, high activity | Pseudopedobacter saltans JCM 21818 | ? | - |
? | |
xylan + H2O | molecular weight of 20000-30000, high activity | Pseudopedobacter saltans NCIMB 13643 | ? | - |
? | |
xylan + H2O | molecular weight of 20000-30000, high activity | Pseudopedobacter saltans ATCC 51119 | ? | - |
? | |
xylan + H2O | molecular weight of 20000-30000, high activity | Pseudopedobacter saltans LMG 10337 | ? | - |
? | |
xylan + H2O | molecular weight of 20000-30000, high activity | Pseudopedobacter saltans DSM 12145 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Pseudopedobacter saltans |
Synonyms | Comment | Organism |
---|---|---|
beta-1,4-xylanase | - |
Pseudopedobacter saltans |
endo-acting beta-1,4-xylanase | - |
Pseudopedobacter saltans |
Pedsa_2704 | - |
Pseudopedobacter saltans |
PsGH10A | - |
Pseudopedobacter saltans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudopedobacter saltans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 55 | recombinant enzyme, activity range, profile overview | Pseudopedobacter saltans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzyme, pH 6.0, 90 min, stable up to | Pseudopedobacter saltans |
45 | - |
purified recombinant enzyme, pH 6.0, 90 min, loss of 50% activity | Pseudopedobacter saltans |
50 | - |
purified recombinant enzyme, pH 6.0, 90 min, loss of 70% activity | Pseudopedobacter saltans |
60 | - |
purified recombinant enzyme, pH 6.0, 90 min, inactivation | Pseudopedobacter saltans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.8 | - |
birchwood xylan | His-tagged enzyme, pH 6.0, 40°C | Pseudopedobacter saltans | |
45.9 | - |
xylan | His-tagged enzyme, pH 6.0, 40°C, substrate xylan MW 20000-30000 | Pseudopedobacter saltans | |
52.2 | - |
beechwood xylan | His-tagged enzyme, pH 6.0, 40°C | Pseudopedobacter saltans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudopedobacter saltans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | recombinant enzyme, activity range, profile overview | Pseudopedobacter saltans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
purified recombinant enzyme, 25°C, 90 min, 25% activity remaining | Pseudopedobacter saltans |
5.5 | - |
purified recombinant enzyme, 25°C, 90 min, 50% activity remaining | Pseudopedobacter saltans |
6 | 8 | purified recombinant enzyme, 25°C, 90 min, over 80% activity remaining | Pseudopedobacter saltans |
8.5 | - |
purified recombinant enzyme, 25°C, 90 min, 50% activity remaining | Pseudopedobacter saltans |
9 | - |
purified recombinant enzyme, 25°C, 90 min, 25% activity remaining | Pseudopedobacter saltans |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 10, GH10 | Pseudopedobacter saltans |