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Literature summary for 3.2.1.8 extracted from
- A novel member of family 30 glycoside hydrolase subfamily 8 glucuronoxylan endo-beta-1,4-xylanase (CtXynGH30) from Clostridium thermocellum orchestrates catalysis on arabinose decorated xylans (2016), J. Mol. Catal. B, 129, 6-14 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Acetivibrio thermocellus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| CtXynGH30, recombinant overexpression in Escherichia coli strain BL21 | Acetivibrio thermocellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Acetivibrio thermocellus | |
| Ca2+ | slight inhibition | Acetivibrio thermocellus | |
| Co2+ | - |
Acetivibrio thermocellus | |
| Cu2+ | - |
Acetivibrio thermocellus | |
| Fe2+ | - |
Acetivibrio thermocellus | |
| Hg2+ | complete inhibition at 1 mM | Acetivibrio thermocellus | |
| Mg2+ | slight inhibition | Acetivibrio thermocellus | |
| NaCl | - |
Acetivibrio thermocellus | |
| Ni2+ | - |
Acetivibrio thermocellus | |
| SDS | - |
Acetivibrio thermocellus | |
| Urea | - |
Acetivibrio thermocellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Vmax and Km are 40.52 U/mg and 2.2 mg/ml, respectively, with beechwood xylan as substrate | Acetivibrio thermocellus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | activates | Acetivibrio thermocellus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-methyl glucuronoxylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| arabinan + H2O | from sugar beet, low activity | Acetivibrio thermocellus | ? | - |
? | |
| arabinogalactan + H2O | low activity | Acetivibrio thermocellus | ? | - |
? | |
| arabinoxylan + H2O | from rye or wheat, low activity | Acetivibrio thermocellus | ? | - |
? | |
| beechwood xylan + H2O | best substrate, TLC analysis of CtXynGH30 hydrolysis products from beechwood xylan. CtXynGH30 shows the release of a series of higher xylooligosaccharides, overview | Acetivibrio thermocellus | ? | - |
? | |
| birchwood xylan + H2O | high activity | Acetivibrio thermocellus | ? | - |
? | |
| galactomannan + H2O | from Konjac, low activity | Acetivibrio thermocellus | ? | - |
? | |
| galactomannan + H2O | from locust bean, low activity | Acetivibrio thermocellus | ? | - |
? | |
| additional information | CtXynGH30 shows the release of a series of higher xylooligosaccharides, implying the endo-acting cleavageby the enzyme. No activity on 4-nitrophenyl-beta-D-xylopyranoside indicating that CtXynGH30 lacks exo-cleaving activity. No activity with carob galactomannan, galactan (lupin), beta-D-glucan (barley), carboxy methylcellulose, pecticgalactan (apple), pecticgalactan (Citrus), pecticgalactan (lupin), rhamnogalacturonan, curdlan, pullulan, and pustulan | Acetivibrio thermocellus | ? | - |
? | |
| oat spelt xylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| xyloglucan + H2O | low activity | Acetivibrio thermocellus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, recombinant enzyme, SDS-PAGE, x * 61200, about, sequence calculation | Acetivibrio thermocellus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CtXynGH30 | - |
Acetivibrio thermocellus |
| family 30 glycoside hydrolase subfamily 8 glucuronoxylan endo-beta-1,4-xylanase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Acetivibrio thermocellus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 85 | over 50% of maximal activity within this range, profile overview | Acetivibrio thermocellus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2008.3 | - |
beechwood xylan | pH 6.0, 37°C | Acetivibrio thermocellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Acetivibrio thermocellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 11.5 | activity range, profile overview | Acetivibrio thermocellus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | completely stable at pH 7.0, over 50% activity remaining at pH 4.0-11.0, inactivation at pH 12.0 and pH below 2.0 | Acetivibrio thermocellus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | CtXynGH30 is a family 30 glycoside hydrolase subfamily 8 glucuronoxylan endo-beta-1,4-xylanase | Acetivibrio thermocellus |
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