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Literature summary for 3.2.1.8 extracted from

  • Cai, L.; Zhang, M.; Shao, T.; He, Y.; Li, J.; Ren, B.; Zhou, C.
    Effect of introducing disulfide bridges in C-terminal structure on the thermostability of xylanase XynZF-2 from Aspergillus niger (2019), J. Gen. Appl. Microbiol., 65, 240-245.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA activates Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
the mutant gene xynZFTA is cloned into pPIC9K and expressed in Pichia pastoris Aspergillus niger

Protein Variants

Protein Variants Comment Organism
T205C/A52C the optimum temperature of the mutant enzyme is improved from 45°C to 60°C, and it retains greater than 90.0% activity (wild-type enzyme retains only 50.0% activity) after treatment at 50°C for 85 min. The optimum pH of mutant xylanase is similar to wild-type enzyme (pH 5.0). The pH stability span (5.0-7.0) of the wild-type enzyme is increased to 3.0-9.0 for the mutant enzyme Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Aspergillus niger
Fe3+
-
Aspergillus niger
Mn2+
-
Aspergillus niger
Pb2+
-
Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Aspergillus niger
Zn2+ activates Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger I3QKR9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger

Synonyms

Synonyms Comment Organism
xylanase XynZF-2
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
wild-type enzyme Aspergillus niger
60
-
mutant enzyme T205C/A52C Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
1 h, the wild-type enzyme almost completely loses its activity Aspergillus niger
55
-
1 h, mutant enzyme T205C/A52C retains more than 80% of its initial activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
wild-type enzyme and mutant enzyme T205C/A52C Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 1 h, 40°C, mutant enzyme T205C/A52C is stable Aspergillus niger
5 7 1 h, 40°C, wild-type enzyme is stable Aspergillus niger