Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | activates | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
the mutant gene xynZFTA is cloned into pPIC9K and expressed in Pichia pastoris | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
T205C/A52C | the optimum temperature of the mutant enzyme is improved from 45°C to 60°C, and it retains greater than 90.0% activity (wild-type enzyme retains only 50.0% activity) after treatment at 50°C for 85 min. The optimum pH of mutant xylanase is similar to wild-type enzyme (pH 5.0). The pH stability span (5.0-7.0) of the wild-type enzyme is increased to 3.0-9.0 for the mutant enzyme | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Aspergillus niger | |
Fe3+ | - |
Aspergillus niger | |
Mn2+ | - |
Aspergillus niger | |
Pb2+ | - |
Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Aspergillus niger | |
Zn2+ | activates | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | I3QKR9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
xylanase XynZF-2 | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
wild-type enzyme | Aspergillus niger |
60 | - |
mutant enzyme T205C/A52C | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
1 h, the wild-type enzyme almost completely loses its activity | Aspergillus niger |
55 | - |
1 h, mutant enzyme T205C/A52C retains more than 80% of its initial activity | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
wild-type enzyme and mutant enzyme T205C/A52C | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | 1 h, 40°C, mutant enzyme T205C/A52C is stable | Aspergillus niger |
5 | 7 | 1 h, 40°C, wild-type enzyme is stable | Aspergillus niger |