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Literature summary for 3.2.1.8 extracted from
- Bi-functional fusion enzyme (EG-M-Xyn)EG-M-Xyn displaying endoglucanase and xylanase activities and its utility in improving lignocellulose degradation (2018), Int. J. Biol. Macromol., 111, 722-729 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetone | 5%, 4.3fold activation of activity with carboxymethyl cellulose, slight activation of xylanase activity (to 104.6% of control), fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |  |
| butanol | 5%, 1.1fold activation of activity with carboxymethyl cellulose, 73% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| EDTA | 5 mM, 90.6% inhibition of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| glycerol | 5%, 8.2fold activation of activity with carboxymethyl cellulose, 10% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| SDS | 1%, 5.7fold activation of activity with carboxymethyl cellulose, 4.5fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
Application
| Application | Comment | Organism |
|---|---|---|
| biofuel production | the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus has great potential in generating fermentable sugars from renewable agro-residues for biofuel and fine chemical industry. Application of the fusion enzyme (EG-M-Xyn)in combination with Ctec2 (commercial enzyme) in the saccharification leads to a 10-20% net increase in fermentable sugars liberated from pretreated rice straw in comparison to the Ctec2 alone | Thermomyces lanuginosus |
| industry | the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus has great potential in generating fermentable sugars from renewable agro-residues for biofuel and fine chemical industry. Application of the fusion enzyme (EG-M-Xyn)in combination with Ctec2 (commercial enzyme) in the saccharification leads to a 10-20% net increase in fermentable sugars liberated from pretreated rice straw in comparison to the Ctec2 alone | Thermomyces lanuginosus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus in Yarrowia lipolytica | Thermomyces lanuginosus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 5 mM, 1.6fold activation of activity with carboxymethyl cellulose, 16.4% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| EDTA | 5 mM, 90.6% inhibition of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| ethanol | 5%, 16% inhibition of activity with carboxymethyl cellulose, 24% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Fe2+ | 5 mM, 2.9fold activation of activity with carboxymethyl cellulose, 6.7% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Fe3+ | 5 mM, 1.1fold activation of activity with carboxymethyl cellulose, 9.1% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Isopropanol | 5%, 21.6% inhibition of activity with carboxymethyl cellulose, 53% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| K+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 13% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| methanol | 5%, 26.5% inhibition of activity with carboxymethyl cellulose, 13.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Mg2+ | 5 mM, 2.3fold activation of activity with carboxymethyl cellulose, 6.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Na+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 6% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| NaN3 | 0.1%, 45.2% inhibition of activity with carboxymethyl cellulose, 12.25% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethyl cellulose | Km-value: 6.8 mg/ml, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, pH 5.0, 70°C | Thermomyces lanuginosus | |
| additional information | - |
beechwood xylan | Km-value: 60.6 mg/ml, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, pH 5.0, 70°C | Thermomyces lanuginosus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Co2+ | 5 mM, 3.3fold activation of activity with carboxymethyl cellulose, 1.4fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Cu2+ | 5 mM, 1.6fold activation of activity with carboxymethyl cellulose, 16.4% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Fe2+ | 5 mM, 2.9fold activation of activity with carboxymethyl cellulose, 6.7% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Fe3+ | 5 mM, 1.1fold activation of activity with carboxymethyl cellulose, 9.1% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| K+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 13% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Mg2+ | 5 mM, 2.3fold activation of activity with carboxymethyl cellulose, 6.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Mn2+ | 5 mM, 6.6fold activation of activity with carboxymethyl cellulose, 1.6fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Na+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 6% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| Zn2+ | 5 mM, 1.7fold activation of activity with carboxymethyl cellulose, no effect on xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75500 | - |
gel filtration, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomyces lanuginosus | O43097 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| avicel + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | ? | - |
? | |
| barley glucan + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | ? | - |
? | |
| beechwood xylan + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | ? | - |
? | |
| birchwood xylan + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | ? | - |
? | |
| carboxymethyl cellulose + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
carboxymethyl cellulase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus and of the parental endoglucanase | Thermomyces lanuginosus |
| 65 | - |
parental xylanase | Thermomyces lanuginosus |
| 70 | - |
xylanase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
t1/2: 173 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Thermomyces lanuginosus |
| 50 | - |
t1/2: 693 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Thermomyces lanuginosus |
| 60 | - |
t1/2: 16 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Thermomyces lanuginosus |
| 60 | - |
t1/2: 347 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Thermomyces lanuginosus |
| 70 | - |
t1/2: 15 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Thermomyces lanuginosus |
| 70 | - |
t1/2: 231 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Thermomyces lanuginosus |
| 80 | - |
t1/2: 12 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Thermomyces lanuginosus |
| 80 | - |
t1/2: 63 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Thermomyces lanuginosus |
| 90 | - |
t1/2: 12 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Thermomyces lanuginosus |
| 90 | - |
t1/2: 20 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Thermomyces lanuginosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
carboxymethyl cellulase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus and of the parental endoglucanase | Thermomyces lanuginosus |
| 7 | - |
xylanase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |
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