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Literature summary for 3.2.1.8 extracted from

  • de Camargo, B.R.; Claassens, N.J.; Quirino, B.F.; Noronha, E.F.; Kengen, S.W.M.
    Heterologous expression and characterization of a putative glycoside hydrolase family 43 arabinofuranosidase from Clostridium thermocellum B8 (2018), Enzyme Microb. Technol., 109, 74-83 .
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acetivibrio thermocellus

Inhibitors

Inhibitors Comment Organism Structure
K+ about 80% residual activity at 20 mM Acetivibrio thermocellus
Mg2+ about 77% residual activity at 20 mM Acetivibrio thermocellus
Na+ about 70% residual activity at 20 mM Acetivibrio thermocellus
Ni2+ about 60% residual activity at 20 mM Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 4-nitrophenyl alpha-L-arabinofuranoside at pH 5.0 and 50°C Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ more than 70% increase of activity at 20 mM Acetivibrio thermocellus
Fe3+ more than 20% increase of activity at 20 mM Acetivibrio thermocellus
additional information Zn2+ and Mn2+ show almost no effect on enzyme activity Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
wheat arabinoxylan + H2O Acetivibrio thermocellus
-
 xylose + ?
-
 ?
wheat arabinoxylan + H2O Acetivibrio thermocellus B8
-
 xylose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Acetivibrio thermocellus B8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Mono-Q column chromatography Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Acetivibrio thermocellus 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Acetivibrio thermocellus B8 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O highest activity Acetivibrio thermocellus 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O highest activity Acetivibrio thermocellus B8 4-nitrophenol + beta-D-xylopyranose
-
 ?
additional information the enzyme does not present activity against arabinan, oat spelt xylan, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, and 4-nitrophenyl alpha L-arabinopyranoside Acetivibrio thermocellus ?
-
 ?
additional information the enzyme does not present activity against arabinan, oat spelt xylan, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, and 4-nitrophenyl alpha L-arabinopyranoside Acetivibrio thermocellus B8 ?
-
 ?
wheat arabinoxylan + H2O
-
 Acetivibrio thermocellus xylose + ?
-
 ?
wheat arabinoxylan + H2O
-
 Acetivibrio thermocellus B8 xylose + ?
-
 ?

Synonyms

Synonyms Comment Organism
AxB8
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Acetivibrio thermocellus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 60 more than 50% activity between 37 and 60°C Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 the enzyme activity remains stable after 16 h at 50°C. At 60°C, the enzyme shows less than 40% activity after 3 h Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
104
-
 4-nitrophenyl alpha-L-arabinofuranoside at pH 5.0 and 50°C Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Acetivibrio thermocellus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.5
-
 4-nitrophenyl alpha-L-arabinofuranoside at pH 5.0 and 50°C Acetivibrio thermocellus