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Literature summary for 3.2.1.8 extracted from
- Kinetic and thermodynamic study of cloned thermostable endo-1,4-beta-xylanase from Thermotoga petrophila in mesophilic host (2012), Mol. Biol. Rep., 39, 7251-7261 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21 Codon plus | Thermotoga petrophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 11% inhibition | Thermotoga petrophila |  |
| Cu2+ | 1 mM, 20% inhibition | Thermotoga petrophila | |
| Hg2+ | 1 mM, 50% inhibition | Thermotoga petrophila | |
| Mn2+ | 1 mM, 71% inhibition | Thermotoga petrophila | |
| additional information | the enzyme shows maximum activity even in the presence of di/trivalent metal cations Ca2+, Cd2+, Pb2+, Mg2+, Ni2+ and Fe3+ | Thermotoga petrophila | |
| Zn2+ | 1 mM, 21% inhibition | Thermotoga petrophila | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
birchwood xylan | pH 6.0, 95°C, Km-value: 3.5 mg/ml | Thermotoga petrophila |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 1 mM, substrate: birchwood xylan | Thermotoga petrophila | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE | Thermotoga petrophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga petrophila | A5IL00 | - |
- |
| Thermotoga petrophila DSM 13995 | A5IL00 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga petrophila |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2600 | - |
pH 6.0, 95°C, substrate, birchwood xylan | Thermotoga petrophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | minor activity | Thermotoga petrophila | ? | - |
? | |
| barley beta-glucan + H2O | minor activity | Thermotoga petrophila DSM 13995 | ? | - |
? | |
| beechwood xylan + H2O | - |
Thermotoga petrophila | ? | - |
? | |
| beechwood xylan + H2O | - |
Thermotoga petrophila DSM 13995 | ? | - |
? | |
| birchwood xylan + H2O | - |
Thermotoga petrophila | ? | - |
? | |
| birchwood xylan + H2O | - |
Thermotoga petrophila DSM 13995 | ? | - |
? | |
| carboxylmethyl cellulose + H2O | minor activity | Thermotoga petrophila | ? | - |
? | |
| carboxylmethyl cellulose + H2O | minor activity | Thermotoga petrophila DSM 13995 | ? | - |
? | |
| additional information | no activity against Avicel, starch, laminarin and Whatman filter paper 42 is observed | Thermotoga petrophila | ? | - |
? | |
| additional information | no activity against Avicel, starch, laminarin and Whatman filter paper 42 is observed | Thermotoga petrophila DSM 13995 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tpet_0854 | - |
Thermotoga petrophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Thermotoga petrophila |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 100 | 70°C: about 40% of maximal activity, 100°C: about 70% of maximal activity | Thermotoga petrophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
8 h, enzyme remains stable | Thermotoga petrophila |
| 96 | - |
half-life: 54.5 min | Thermotoga petrophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
at 70°C | Thermotoga petrophila |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | pH 3.0: about 35% of maximal activity, pH 11.0: about 40% of maximal activity | Thermotoga petrophila |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
reduction to about 20% residual enzymatic activity | Thermotoga petrophila |
| 4 | 7 | the enzyme retains residual enzymatic activity of greater than 50% at pH 4.0-7.0 | Thermotoga petrophila |
| 6 | - |
stable at pH 6.0 (citrate phosphate buffer) | Thermotoga petrophila |
| 10 | - |
reduction to about 20% residual enzymatic activity | Thermotoga petrophila |
| 11 | - |
reduction to about 20% residual enzymatic activity | Thermotoga petrophila |
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