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Literature summary for 3.2.1.8 extracted from

  • de Queiroz Brito Cunha, C.C.; Gama, A.R.; Cintra, L.C.; Bataus, L.A.M.; Ulhoa, C.J.
    Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris (2018), PLoS ONE, 13, e0192996 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 5 mM, 152% of initial activity Streptomyces sp.

Application

Application Comment Organism
food industry use of enzyme as an additive in the bread making process leads to a decrease in firmness, stiffness and consistency, and improvements in specific volume and reducing sugar content Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 5 mM, no residual activity Streptomyces sp.
Cu2+ 5 mM, 72% residual activity Streptomyces sp.
Hg2+ 5 mM, 34% residual activity Streptomyces sp.
Mn2+ 5 mM, 54% residual activity Streptomyces sp.
SDS 5 mM, 51% residual activity Streptomyces sp.
xylose 40 mM, 81% residual activity Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 beechwood xylan Km value 12.4 mg/ml, pH 6.0, 50°C Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 5 mM, 142% of initial activity Streptomyces sp.
Mg2+ 5 mM, 135% of initial activity Streptomyces sp.
NH4Cl 5 mM, 142% of initial activity Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. D1FNQ6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
 Streptomyces sp. ?
-
 ?
oat spelt xylan + H2O
-
 Streptomyces sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 20000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
XynBS27
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 more than 60% of maximum activity Streptomyces sp.