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Literature summary for 3.2.1.8 extracted from

  • Wu, H.; Cheng, X.; Zhu, Y.; Zeng, W.; Chen, G.; Liang, Z.
    Purification and characterization of a cellulase-free, thermostable endo-xylanase from Streptomyces griseorubens LH-3 and its use in biobleaching on eucalyptus kraft pulp (2018), J. Biosci. Bioeng., 125, 46-51 .
    View publication on PubMed

Application

Application Comment Organism
degradation application of enzyme for biobleaching of Eucalyptus kraft pulp, the xylanase increases the brightness of the pulp by 14.5% and reduces the kappa number by 24.5% Streptomyces griseorubens

Cloned(Commentary)

Cloned (Comment) Organism
-
Streptomyces griseorubens

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 5 mM, 25% of initial activity Streptomyces griseorubens
Ca2+ 5 mM, 76% of initial activity Streptomyces griseorubens
Cu2+ 5 mM, 73% of initial activity Streptomyces griseorubens
Hg2+ 5 mM, 8% of initial activity Streptomyces griseorubens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
birchwood xylan Km value 1.44 mg/ml, pH 5.0, 50°C Streptomyces griseorubens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 5 mM, 113% of initial activity Streptomyces griseorubens
Mg2+ 5 mM, 110% of initial activity Streptomyces griseorubens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
gel filtration Streptomyces griseorubens

Organism

Organism UniProt Comment Textmining
Streptomyces griseorubens
-
-
-
Streptomyces griseorubens LH-3
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
434
-
pH 5.0, 50°C Streptomyces griseorubens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O
-
Streptomyces griseorubens ?
-
?
birchwood xylan + H2O
-
Streptomyces griseorubens LH-3 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45500, SDS-PAGE Streptomyces griseorubens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Streptomyces griseorubens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
1 h, 60% residual activity Streptomyces griseorubens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Streptomyces griseorubens

pH Range

pH Minimum pH Maximum Comment Organism
4 8 more than 80% of maxiumum activity Streptomyces griseorubens