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Literature summary for 3.2.1.8 extracted from
- Purification and characterization of xylanases from Thermomyces lanuginosus and its mutant derivative possessing novel kinetic and thermodynamic properties (2009), World J. Microbiol. Biotechnol., 25, 493-502 .
No PubMed abstract available
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | random mutagenesis leads to mutant M7 with improved kinetic and thermodynamic properties | Thermomyces lanuginosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
oat spelt xylan | Km value of wild-type 0.112 mg/ml, of mutant M7 0.098 mg/ml, respectively, pH 6.0, 50°C | Thermomyces lanuginosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
gel filtration | Thermomyces lanuginosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomyces lanuginosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermomyces lanuginosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15501 | - |
wild-type, pH 6.0, 50°C | Thermomyces lanuginosus |
| 17778 | - |
mutant M7, pH 6.0, 50°C | Thermomyces lanuginosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oat spelt xylan + H2O | - |
Thermomyces lanuginosus | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 24000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
wild-type | Thermomyces lanuginosus |
| 70 | - |
mutant M7 | Thermomyces lanuginosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5967 | - |
oat spelt xylan | wild-type, pH 6.0, 50°C | Thermomyces lanuginosus | |
| 7159 | - |
oat spelt xylan | mutant M7, pH 6.0, 50°C | Thermomyces lanuginosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
wild-type | Thermomyces lanuginosus |
| 8.1 | - |
mutant M7 | Thermomyces lanuginosus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
oat spelt xylan | kcat/Km value of wild-type 53279 ml/mg/s, of mutant M7 73048 ml/mg/s, respectively, pH 6.0, 50°C | Thermomyces lanuginosus |
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