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Literature summary for 3.2.1.8 extracted from

  • Hoffmam, Z.; Zanphorlin, L.; Cota, J.; Diogo, J.; Almeida, G.; Damasio, A.; Squina, F.; Murakami, M.; Ruller, R.
    Xylan-specific carbohydrate-binding module belonging to family 6 enhances the catalytic performance of a GH11 endo-xylanase (2016), New Biotechnol., 33, 467-472 .
    View publication on PubMed
Search for more literature for 3.2.1.8

Application

Application Comment Organism
degradation when a fusion protein with the carbohydrate-binding domain of xylanase XynZ from Clostridium thermocellum supplements the commercial cocktail Accellerase1 1500, reducing sugar release is improved by 17% from pretreated sugarcane bagasse Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information fusion of enzyme with a carbohydrate-binding module from Clostridium thermocellum which exhibits high affinity to xylan. The molecular fusion does not alter the pH and temperature dependence, but leads to an increase of 65% in the catalytic efficiency. As supplement in the commercial cocktail Accellerase1 1500, the chimeric enzyme improves the reducing sugar release by 17% from pretreated sugarcane bagasse Bacillus subtilis
additional information construction of a fusion protein with the carbohydrate-binding doamin of xylanase XynZ from Clostridium thermocellum. The fusion does not alter the pH and temperature dependence, but increases the catalytic activity by 65% Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 beechwood xylan Km value for wild-type 5.84 mg/ml, for chimera carrying a carbohydrate-binding module 4.04 mg/ml, respectively, pH 6.0, 50°C Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P18429
-
-
Bacillus subtilis 168 P18429
-
-

Purification (Commentary)

Purification (Comment) Organism
from wheat malt Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.52
-
 substrate rye arabinoxylan xylan, wild-type, pH 6.0, 50°C Bacillus subtilis
0.67
-
 substrate oat spelt xylan, wild-type, pH 6.0, 50°C Bacillus subtilis
0.87
-
 substrate rye arabinoxylan xylan, fusion protein with XylZ CBM domain, pH 6.0, 50°C Bacillus subtilis
0.88
-
 substrate oat spelt xylan, fusion protein with XylZ CBM domain, pH 6.0, 50°C Bacillus subtilis
2.58
-
 substrate beechwood xylan, wild-type, pH 6.0, 50°C Bacillus subtilis
3.03
-
 substrate beechwood xylan, fusion protein with XylZ CBM domain, pH 6.0, 50°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
 Bacillus subtilis ?
-
 ?
beechwood xylan + H2O
-
 Bacillus subtilis 168 ?
-
 ?
oat spelt xylan + H2O
-
 Bacillus subtilis ?
-
 ?
oat spelt xylan + H2O
-
 Bacillus subtilis 168 ?
-
 ?
rye arabinoxylan + H2O
-
 Bacillus subtilis ?
-
 ?
rye arabinoxylan + H2O
-
 Bacillus subtilis 168 ?
-
 ?

Synonyms

Synonyms Comment Organism
Xyl11
-
 Bacillus subtilis
XynA
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
237
-
 beechwood xylan wild-type, pH 6.0, 50°C Bacillus subtilis
268
-
 beechwood xylan chimera carrying a carbohydrate-binding module, pH 6.0, 50°C Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 beechwood xylan kcat/Km value for wild-type 40.6 ml/mg/s, for chimera carrying a carbohydrate-binding module 66.4 ml/mg/s, respectively, pH 6.0, 50°C Bacillus subtilis