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Literature summary for 3.2.1.8 extracted from

  • Triches Damaso, M.; Carvalho Andrade, C.; Pereira Jr., N.
    Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 (2002), Braz. J. Microbiol., 33, 333-338 .
No PubMed abstract available
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 5 mM, 140% of initial activity Thermomyces lanuginosus
dithiothreitol 5 mM, 113% of initial activity Thermomyces lanuginosus

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 5 mM, 88% residual activity Thermomyces lanuginosus
Fe3+ 5 mM, 78% residual activity Thermomyces lanuginosus
Mg2+ 5 mM, 74% residual activity Thermomyces lanuginosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermomyces lanuginosus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 5 mM, 113% of initial activity Thermomyces lanuginosus
Na+ 5 mM, 110% of initial activity Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
-
-
Thermomyces lanuginosus IOC-4145
-
-
-

Storage Stability

Storage Stability Organism
-20°C, crude culture, 30 days, 15-20% loss of activity, maintaining stable thereafter for 5 months Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O 77% of the activtiy with oat spelt xylan Thermomyces lanuginosus ?
-
 ?
beechwood xylan + H2O 77% of the activtiy with oat spelt xylan Thermomyces lanuginosus IOC-4145 ?
-
 ?
birchwood xylan + H2O 84% of the activtiy with oat spelt xylan Thermomyces lanuginosus ?
-
 ?
birchwood xylan + H2O 84% of the activtiy with oat spelt xylan Thermomyces lanuginosus IOC-4145 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Thermomyces lanuginosus ?
-
 ?
carboxymethyl cellulose + H2O
-
 Thermomyces lanuginosus IOC-4145 ?
-
 ?
oat spelt xylan + H2O
-
 Thermomyces lanuginosus ?
-
 ?
oat spelt xylan + H2O
-
 Thermomyces lanuginosus IOC-4145 ?
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75
-
 assay at Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 24 h, 50% residual activity Thermomyces lanuginosus
60
-
 4 h, 50% residual activity Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Thermomyces lanuginosus

General Information

General Information Comment Organism
physiological function maximum peaks of xylanase production and specific activity occurr after 96 hours of cultivation in liquid medium using 3% (w/v) corncob as substrate Thermomyces lanuginosus