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Literature summary for 3.2.1.8 extracted from
- Highly thermostable xylanase of the thermophilic fungus Talaromyces thermophilus purification and characterization (2009), Appl. Biochem. Biotechnol., 158, 200-212 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 10 mM, 65% residual activity | Thermomyces dupontii |  |
| dithiothreitol | 10 mM, 85% residual activity | Thermomyces dupontii | |
| Hg2+ | 10 mM, 65% residual activity | Thermomyces dupontii | |
| Mn2+ | 10 mM, 65% residual activity | Thermomyces dupontii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
birchwood xylan | Km value 22.5 mg/ml, pH 7.0, 50°C | Thermomyces dupontii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermomyces dupontii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 10 mM, 125% of initial activity | Thermomyces dupontii | |
| Co2+ | 10 mM, 112% of initial activity | Thermomyces dupontii | |
| Cu2+ | 10 mM, 105% of initial activity | Thermomyces dupontii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration | Thermomyces dupontii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomyces dupontii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermomyces dupontii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160 | - |
pH 7.0, 50°C | Thermomyces dupontii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| birchwood xylan + H2O | - |
Thermomyces dupontii | ? | - |
? | |
| oat spelt xylan + H2O | - |
Thermomyces dupontii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 25000, SDS-PAGE | Thermomyces dupontii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermomyces dupontii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable for 7 days | Thermomyces dupontii |
| 100 | - |
half-life 60 min | Thermomyces dupontii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Thermomyces dupontii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
80% of maximum activity | Thermomyces dupontii |
| 9 | - |
85% of maximum activity | Thermomyces dupontii |
| 10 | - |
60% of maximum activity | Thermomyces dupontii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | highest titer of xylanase (5.5 IU/ml) is obtained b y growth on oat spelt xylan | Thermomyces dupontii |
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Release 2023.1 (January 2023)
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