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Literature summary for 3.2.1.8 extracted from

  • Mitra, S.; Mukhopadhyay, B.; Mandal, A.; Arukha, A.; Chakrabarty, K.; Das, G.; Chakrabartty, P.; Biswas, S.
    Cloning, overexpression, and characterization of a novel alkali-thermostable xylanase from Geobacillus sp. WBI (2015), J. Basic Microbiol., 55, 527-537.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus sp. WBI

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
birchwood xylan Km value 0.9 mg/ml, pH 7, 65°C Geobacillus sp. WBI

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
x * 47000, SDS-PAGE Geobacillus sp. WBI

Organism

Organism UniProt Comment Textmining
Geobacillus sp. WBI B5M201
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O
-
Geobacillus sp. WBI xylo-oligosaccharides + ?
-
?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Geobacillus sp. WBI

Synonyms

Synonyms Comment Organism
XYLA
-
Geobacillus sp. WBI

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
1 h, pH 10, 100% residual activity Geobacillus sp. WBI

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
Geobacillus sp. WBI

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
1 h, 65°C, 100% residual activity Geobacillus sp. WBI
11
-
1 h, 65°C, 75% residual activity Geobacillus sp. WBI