Any feedback?
Literature summary for 3.2.1.8 extracted from
- Calcium alginate matrix increases the stability and recycling capability of immobilized endo-beta-1,4-xylanase from Geobacillus stearothermophilus KIBGE-IB29 (2015), Extremophiles, 19, 819-827.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | immobilization of enzyme within calcium alginate beads using entrapment technique. Temperature (50°C) and pH (7.0) optima of immobilized enzyme remain same, but enzyme-substrate reaction time increases from 5.0 to 30.0 min as compared to free enzyme. The diffusion limit of high molecular weight xylan (corncob) causes a decline in Vmax of immobilized enzyme from 4773 to 203.7 U/min, whereas the Km value increases from 0.5074 to 0.5722 mg/ml. Immobilized endo-beta-1,4-xylanase is stable even at high temperatures and retains 18 and 9% residual activity at 70°C and 80°C, respectively. The immobilized enzyme also exhibits sufficient recycling efficiency up to five reaction cycles | Geobacillus stearothermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
corncob xylan | Km value of free enzyme is 0.507 mg/ml, of immobilized enzyme 0.572 mg/ml, pH 7.0, 50°C | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus KIBGE-IB29 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| corncob xylan + H2O | - |
Geobacillus stearothermophilus | xylo-oligosaccharides + ? | - |
? |  |
| corncob xylan + H2O | - |
Geobacillus stearothermophilus KIBGE-IB29 | xylo-oligosaccharides + ? | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
18% of maximum activity | Geobacillus stearothermophilus |
| 80 | - |
9% of maximum activity | Geobacillus stearothermophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
