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Literature summary for 3.2.1.8 extracted from

  • Sermsathanaswadi, J.; Baramee, S.; Tachaapaikoon, C.; Pason, P.; Ratanakhanokchai, K.; Kosugi, A.
    The family 22 carbohydrate-binding module of bifunctional xylanase/beta-glucanase Xyn10E from Paenibacillus curdlanolyticus B-6 has an important role in lignocellulose degradation (2017), Enzyme Microb. Technol., 96, 75-84.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paenibacillus curdlanolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
soluble birchwood xylan Km value 10.4 mg/ml, pH 6.0, temperature not specified in the publication Paenibacillus curdlanolyticus

Organism

Organism UniProt Comment Textmining
Paenibacillus curdlanolyticus
-
-
-
Paenibacillus curdlanolyticus B-6
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
134
-
substrate insoluble birchwood xylan, pH 6.0, temperature not specified in the publication Paenibacillus curdlanolyticus
1487
-
substrate soluble birchwood xylan, pH 6.0, temperature not specified in the publication Paenibacillus curdlanolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
insoluble birchwood xylan + H2O
-
Paenibacillus curdlanolyticus xylo-oligosaccharides + ?
-
?
insoluble birchwood xylan + H2O
-
Paenibacillus curdlanolyticus B-6 xylo-oligosaccharides + ?
-
?
additional information enzyme shows bifunctional degradation activity toward xylan and beta-glucan and also degrades microcrystalline cellulose Paenibacillus curdlanolyticus ?
-
?
additional information enzyme shows bifunctional degradation activity toward xylan and beta-glucan and also degrades microcrystalline cellulose Paenibacillus curdlanolyticus B-6 ?
-
?
soluble birchwood xylan + H2O
-
Paenibacillus curdlanolyticus xylo-oligosaccharides + ?
-
?
soluble birchwood xylan + H2O
-
Paenibacillus curdlanolyticus B-6 xylo-oligosaccharides + ?
-
?

Synonyms

Synonyms Comment Organism
Xyn10E
-
Paenibacillus curdlanolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
129
-
soluble birchwood xylan pH 6.0, temperature not specified in the publication Paenibacillus curdlanolyticus

General Information

General Information Comment Organism
physiological function enzyme consists of a family 22 carbohydrate-binding module, a glycoside hydrolase family 10 catalytic domain, two fibronectin type III (Fn3) domains, and a family 3 carbohydrate-binding module at the C-terminus. The family 22 carbohydrate-binding module has an important role not only in binding to xylan and beta-glucan but also in feeding both polysaccharides into the neighboring GH10 catalytic domain Paenibacillus curdlanolyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
soluble birchwood xylan kcat/Km vlaue 12.3 ml per s and mg, pH 6.0, temperature not specified in the publication Paenibacillus curdlanolyticus