Literature summary for 3.2.1.8 extracted from

Gomez, S.; Payne, A.M.; Savko, M.; Fox, G.C.; Shepard, W.E.; Fernandez, F.J.; Cristina Vega, M.
Structural and functional characterization of a highly stable endo-beta-1,4-xylanase from Fusarium oxysporum and its development as an efficient immobilized biocatalyst (2016), Biotechnol. Biofuels, 9, 191.

Application

Application	Comment	Organism
degradation	immobilizazion of enzyme on various supports. Most active immobilized enzyme is achieved when Xyl2 is covalently bound to low functionalized agarose matrices, poorer activity is observed for Xyl2 immobilized on highly functionalized agarose or on nickel-affinity resin	Fusarium oxysporum f. sp. vasinfectum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Fusarium oxysporum f. sp. vasinfectum

Crystallization (Commentary)

Crystallization (Comment)	Organism
Xyl2 at 1.56 A resolution and in complex with methyl-xylopyranoside at 2.84 A resolution. Xyl2 folds into the characteristic GH11 xylanase fold consisting of a highly twisted beta-sandwich core that comprises 14 beta-strands and is further stabilized by a single alpha-helix in the opposite side of the active site. The hydrogen bonding interactions between methyl-xylopyranoside and residues Glu85, Ser126, Tyr170 and Tyr176 are all located in a cavity encircled by the side chains of Pro5 and the side chains of Trp18, Asn44 and Pro125	Fusarium oxysporum f. sp. vasinfectum

Crystallization (Comment)

Organism

Xyl2 at 1.56 A resolution and in complex with methyl-xylopyranoside at 2.84 A resolution. Xyl2 folds into the characteristic GH11 xylanase fold consisting of a highly twisted beta-sandwich core that comprises 14 beta-strands and is further stabilized by a single alpha-helix in the opposite side of the active site. The hydrogen bonding interactions between methyl-xylopyranoside and residues Glu85, Ser126, Tyr170 and Tyr176 are all located in a cavity encircled by the side chains of Pro5 and the side chains of Trp18, Asn44 and Pro125

Fusarium oxysporum f. sp. vasinfectum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
40	-	4-O-methyl-D-glucurono-D-xylan	pH 8.0, 40°C	Fusarium oxysporum f. sp. vasinfectum

Metals/Ions

Metals/Ions	Comment	Organism
Ca2+	activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+	Fusarium oxysporum f. sp. vasinfectum
Co2+	activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+	Fusarium oxysporum f. sp. vasinfectum
Mg2+	activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+	Fusarium oxysporum f. sp. vasinfectum
Mn2+	activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+	Fusarium oxysporum f. sp. vasinfectum
Ni2+	activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+	Fusarium oxysporum f. sp. vasinfectum
Zn2+	activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+	Fusarium oxysporum f. sp. vasinfectum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21200	-	-	Fusarium oxysporum f. sp. vasinfectum

Organic Solvent Stability

Organic Solvent	Comment	Organism
1-ethyl-3-methylimidazolium acetate	ionic liquid, no inhibition up to 15% w/v	Fusarium oxysporum f. sp. vasinfectum

Organism

Organism	UniProt	Comment	Textmining
Fusarium oxysporum f. sp. vasinfectum	X0M5X0	-	-
Fusarium oxysporum f. sp. vasinfectum 25433	X0M5X0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-O-methyl-D-glucurono-D-xylan + H2O	substrate dyed with Remazol brilliant blue R	Fusarium oxysporum f. sp. vasinfectum	xylo-oligosaccharides + ?	-	?
4-O-methyl-D-glucurono-D-xylan + H2O	substrate dyed with Remazol brilliant blue R	Fusarium oxysporum f. sp. vasinfectum 25433	xylo-oligosaccharides + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 21200, SDS-PAGE	Fusarium oxysporum f. sp. vasinfectum

Synonyms

Synonyms	Comment	Organism
FOTG_15646	-	Fusarium oxysporum f. sp. vasinfectum
XYL2	-	Fusarium oxysporum f. sp. vasinfectum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.27	-	4-O-methyl-D-glucurono-D-xylan	pH 8.0, 40°C	Fusarium oxysporum f. sp. vasinfectum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	8	substrate 4-O-methyl-D-glucurono-D-xylan	Fusarium oxysporum f. sp. vasinfectum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
6.27	-	4-O-methyl-D-glucurono-D-xylan	pH 8.0, 40°C	Fusarium oxysporum f. sp. vasinfectum