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Literature summary for 3.2.1.8 extracted from
- Molecular characterization of xylobiose- and xylopentaose-producing beta-1,4-endoxylanase SCO5931 from Streptomyces coelicolor A3(2) (2016), Appl. Biochem. Biotechnol., 180, 349-360.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Streptomyces lividans TK24 | Streptomyces coelicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 4 mM, 87% inhibition | Streptomyces coelicolor |  |
| additional information | not inhibitory: EDTA, Ca2+, Na+, Ni2+, and Mg2+ | Streptomyces coelicolor |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
beechwood xylan | Km value 0.24 mg/ml, pH 6.0, 50°C | Streptomyces coelicolor |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
- |
Streptomyces coelicolor |
| 47000 | - |
- |
Streptomyces coelicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | Q8CJQ1 | - |
- |
| Streptomyces coelicolor A3(2) | Q8CJQ1 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28940 | - |
pH 6.0, 50°C | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beechwood xylan + H2O | - |
Streptomyces coelicolor | xylobiose + xylopentaose + ? | main products | ? | |
| beechwood xylan + H2O | - |
Streptomyces coelicolor A3(2) | xylobiose + xylopentaose + ? | main products | ? | |
| additional information | no substrates: carboxymethyl cellulose, Avicel, galactan, barley beta-glucan, and xyloglucan | Streptomyces coelicolor | ? | - |
? | |
| additional information | no substrates: carboxymethyl cellulose, Avicel, galactan, barley beta-glucan, and xyloglucan | Streptomyces coelicolor A3(2) | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE, x * 47000, calculated | Streptomyces coelicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SCO5931 | - |
Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Streptomyces coelicolor |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
98% of maximum activity | Streptomyces coelicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, 90% residual activity | Streptomyces coelicolor |
| 60 | - |
1 h, 16% residual activity | Streptomyces coelicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Streptomyces coelicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
40% of maximum activity | Streptomyces coelicolor |
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Release 2023.1 (January 2023)
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