Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arthrobacter sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | truncated derivatives XynAGN16L (truncation of GH 10 domain at N-terminus) and XynAGN16Lpd (truncation of GH 10 domain at N-terminus and polysaccharide deacetylases domain) show similar features, including catalytic activities at 0°C, thermolabilities at temperatures of more than 50°C, and similar substrate specificity. However, the polysaccharide deacetylases domain improves the affinity and catalytic efficiency towards xylans | Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
beechwood xylan | truncated variant XynAGN16L, Km value 1.8 mg/ml, truncated variant 1.0 mg/ml, respcetively, pH 6.5, 37°C | Arthrobacter sp. | |
additional information | - |
birchwood xylan | truncated variant XynAGN16L, Km value 2.6 mg/ml, truncated variant 1.2 mg/ml, respcetively, pH 6.5, 37°C | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
- |
Arthrobacter sp. |
131500 | - |
- |
Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | A0A0F6MV31 | isolated from the feces of Grus nigricollis | - |
Arthrobacter sp. GN16 | A0A0F6MV31 | isolated from the feces of Grus nigricollis | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a putative N-gnal peptide of 25 amino acids | Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
819 | - |
truncated variant XynAGN16Lpd, substrate oat spelt xylan, pH 6.5, 37°C | Arthrobacter sp. |
1081 | - |
truncated variant XynAGN16L, substrate birchwood xylan, pH 6.5, 37°C | Arthrobacter sp. |
1177 | - |
truncated variant XynAGN16Lpd, substrate birchwood xylan, pH 6.5, 37°C | Arthrobacter sp. |
1234 | - |
truncated variant XynAGN16L, substrate beechwood xylan, pH 6.5, 37°C | Arthrobacter sp. |
1242 | - |
truncated variant XynAGN16L, substrate oat spelt xylan, pH 6.5, 37°C | Arthrobacter sp. |
1266 | - |
truncated variant XynAGN16Lpd, substrate beechwood xylan, pH 6.5, 37°C | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beechwood xylan + H2O | - |
Arthrobacter sp. | xylose + xylo-oligosaccharides | - |
? | |
beechwood xylan + H2O | - |
Arthrobacter sp. GN16 | xylose + xylo-oligosaccharides | - |
? | |
birchwood xylan + H2O | - |
Arthrobacter sp. | xylose + xylo-oligosaccharides | - |
? | |
birchwood xylan + H2O | - |
Arthrobacter sp. GN16 | xylose + xylo-oligosaccharides | - |
? | |
oat speltxylan + H2O | - |
Arthrobacter sp. | xylose + xylo-oligosaccharides | - |
? | |
oat speltxylan + H2O | - |
Arthrobacter sp. GN16 | xylose + xylo-oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 131500, calculated, x * 130000, SDS-PAGE | Arthrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
XynA | - |
Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
29% of maximum activity | Arthrobacter sp. |