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Literature summary for 3.2.1.8 extracted from

  • Zhou, J.; Shen, J.; Zhang, R.; Tang, X.; Li, J.; Xu, B.; Ding, J.; Gao, Y.; Xu, D.; Huang, Z.
    Molecular and biochemical characterization of a novel multidomain xylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis (2015), Appl. Biochem. Biotechnol., 175, 573-588.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arthrobacter sp.

Protein Variants

Protein Variants Comment Organism
additional information truncated derivatives XynAGN16L (truncation of GH 10 domain at N-terminus) and XynAGN16Lpd (truncation of GH 10 domain at N-terminus and polysaccharide deacetylases domain) show similar features, including catalytic activities at 0°C, thermolabilities at temperatures of more than 50°C, and similar substrate specificity. However, the polysaccharide deacetylases domain improves the affinity and catalytic efficiency towards xylans Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
beechwood xylan truncated variant XynAGN16L, Km value 1.8 mg/ml, truncated variant 1.0 mg/ml, respcetively, pH 6.5, 37°C Arthrobacter sp.
additional information
-
birchwood xylan truncated variant XynAGN16L, Km value 2.6 mg/ml, truncated variant 1.2 mg/ml, respcetively, pH 6.5, 37°C Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
-
Arthrobacter sp.
131500
-
-
Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. A0A0F6MV31 isolated from the feces of Grus nigricollis
-
Arthrobacter sp. GN16 A0A0F6MV31 isolated from the feces of Grus nigricollis
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a putative N-gnal peptide of 25 amino acids Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
819
-
truncated variant XynAGN16Lpd, substrate oat spelt xylan, pH 6.5, 37°C Arthrobacter sp.
1081
-
truncated variant XynAGN16L, substrate birchwood xylan, pH 6.5, 37°C Arthrobacter sp.
1177
-
truncated variant XynAGN16Lpd, substrate birchwood xylan, pH 6.5, 37°C Arthrobacter sp.
1234
-
truncated variant XynAGN16L, substrate beechwood xylan, pH 6.5, 37°C Arthrobacter sp.
1242
-
truncated variant XynAGN16L, substrate oat spelt xylan, pH 6.5, 37°C Arthrobacter sp.
1266
-
truncated variant XynAGN16Lpd, substrate beechwood xylan, pH 6.5, 37°C Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
Arthrobacter sp. xylose + xylo-oligosaccharides
-
?
beechwood xylan + H2O
-
Arthrobacter sp. GN16 xylose + xylo-oligosaccharides
-
?
birchwood xylan + H2O
-
Arthrobacter sp. xylose + xylo-oligosaccharides
-
?
birchwood xylan + H2O
-
Arthrobacter sp. GN16 xylose + xylo-oligosaccharides
-
?
oat speltxylan + H2O
-
Arthrobacter sp. xylose + xylo-oligosaccharides
-
?
oat speltxylan + H2O
-
Arthrobacter sp. GN16 xylose + xylo-oligosaccharides
-
?

Subunits

Subunits Comment Organism
? x * 131500, calculated, x * 130000, SDS-PAGE Arthrobacter sp.

Synonyms

Synonyms Comment Organism
XynA
-
Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
29% of maximum activity Arthrobacter sp.