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Literature summary for 3.2.1.8 extracted from

  • Guo, N.; Zheng, J.; Tian, J.; Wu, L.; Zhou, H.
    Characterization and constitutive expression of an acidic mesophilic endo-1,4-beta-D-xylanohydrolase with high thermotolerance and catalytic efficiency in Pichia pastoris (2013), World J. Microbiol. Biotechnol., 29, 2095-2103.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
constitutive expression in Pichia pastoris Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
birchwood xylan Km value is 2.95 mg/ml, pH 5.0, 50°C Aspergillus niger
additional information
-
beechwood xylan Km value is 3.0 mg/ml, pH 5.0, 50°C Aspergillus niger
additional information
-
oat spelt xylan Km value is 6.6 mg/ml, pH 5.0, 50°C Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
x * 20000, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22250
-
pH 5.0. 50°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O 85% of the activity with oat spelt xylan Aspergillus niger ?
-
?
birchwood xylan + H2O 64.6% of the activity with oat spelt xylan Aspergillus niger ?
-
?
oat spelt xylan + H2O
-
Aspergillus niger ?
-
?

Subunits

Subunits Comment Organism
? x * 20000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
Xyl11
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 68 more than 68% of maximum activity Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
pH 5.0, 10 min, 40% residual activity Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7764
-
birchwood xylan Km value is 2.95 mg/ml, pH 5.0, 50°C Aspergillus niger
10200
-
beechwood xylan Km value is 3.0 mg/ml, pH 5.0, 50°C Aspergillus niger
17250
-
oat spelt xylan Km value is 6.6 mg/ml, pH 5.0, 50°C Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
5
-
-
Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.2 11 22°C, 2 h, 75% residual activity Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
oat spelt xylan kcat/Km value is 2625 ml per mg and s, pH 5.0, 50°C Aspergillus niger
additional information
-
birchwood xylan kcat/Km value is 2632 ml per mg and s, pH 5.0, 50°C Aspergillus niger
additional information
-
beechwood xylan kcat/Km value is 3401 ml per mg and s, pH 5.0, 50°C Aspergillus niger