Cloned (Comment) | Organism |
---|---|
overexpression in Pichia pastoris | Streptomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant protein expressed in Pichia pastoris without signal peptide exhibits better physicochemical properties than those of the native enzyme including higher optimal temperature of 60°C, and specific activity, but lower optimal pH 4.0 | Streptomyces sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, SDS-PAGE | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | F7J663 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | sequence contains six potential glycosylation sites | Streptomyces sp. |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.3 | - |
native enzyme, pH 6.0, 37°C | Streptomyces sp. |
77.4 | - |
recombinant enzyme, pH 6.0, 37°C | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birchwood xylan + H2O | - |
Streptomyces sp. | ? | - |
? | |
oat spelt xylan + H2O | 52% of the activity with birchwood xylan | Streptomyces sp. | xylobiose + xylotriose + ? | major products | ? | |
wheat arabinoxylan + H2O | 35% of the activity with birchwood xylan | Streptomyces sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
XynSW1 | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to, for 1 h | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 11 | 4°C, 16 h, more than 80% of initial activity | Streptomyces sp. |