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Literature summary for 3.2.1.8 extracted from
- Heterologous expression of endo-1,4-beta-xylanase A from Schizophyllum commune in Pichia pastoris and functional characterization of the recombinant enzyme (2013), Enzyme Microb. Technol., 52, 170-176.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Schizophyllum commune |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, 35% residual activity | Schizophyllum commune |  |
| Fe3+ | 1 mM, 60% residual activity | Schizophyllum commune | |
| Hg2+ | 1 mM, 60% residual activity | Schizophyllum commune | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
beechwood xylan | Km value is 4 mg/ml, pH 5.0, 40°C | Schizophyllum commune |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 M, 125% of initial activity | Schizophyllum commune | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 26000, SDS-PAGE | Schizophyllum commune |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizophyllum commune | P35809 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 316 | - |
substrate arabinoxylan, pH 5.0, 40°C | Schizophyllum commune |
| 5768 | - |
substrate beechwood xylan, pH 5.0, 40°C | Schizophyllum commune |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arabinoxylan xylan + H2O | - |
Schizophyllum commune | ? | - |
? | |
| beechwood xylan + H2O | - |
Schizophyllum commune | ? | - |
? | |
| additional information | no substrates: caboxymethyl cellulose, avicel, filter paper | Schizophyllum commune | ? | - |
? | |
| poplar stem + H2O | - |
Schizophyllum commune | ? | enzyme degrades approximately 45% of unsubstituted xylans in the cell wall from poplar stems | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26000, SDS-PAGE | Schizophyllum commune |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XynA | - |
Schizophyllum commune |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Schizophyllum commune |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
5 min, 40% residual activity | Schizophyllum commune |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Schizophyllum commune |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
70% of maximum activity | Schizophyllum commune |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
18 h, less than 30% of initial activity | Schizophyllum commune |
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Release 2023.1 (January 2023)
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