Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ustilago maydis | Q4P0L3 | - |
- |
Ustilago maydis 521 | Q4P0L3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | - |
Ustilago maydis | xylotriose + ? | after 25 h of reaction only xylotriose is left in the supernatant. No xylobiose or xylose are detected | ? | |
xylan + H2O | - |
Ustilago maydis 521 | xylotriose + ? | after 25 h of reaction only xylotriose is left in the supernatant. No xylobiose or xylose are detected | ? |
Synonyms | Comment | Organism |
---|---|---|
Xyn11A | - |
Ustilago maydis |
General Information | Comment | Organism |
---|---|---|
physiological function | deletion of the gene encoding xylanase 11A reduces the growth rate of the mutant on minimal medium with xylan to 0.09 per h compared to 0.10 per h of the wild-type. The deletion mutant reaches a final OD600 of 6.78 compared to 8.18 in the wild-type culture. The drop of the pH value from initially 5.7 to 3.4 is approximately 3 h delayed in comparison to wild-type. Also the maximal concentration of reducing sugars in the medium is lower, and occurs 9 h later than in the wildtype culture. Heterologous expression of xylanase 11A on the cell surface of a xylanase-negative strain of Saccharomyces cerevisiae enables the strain to almost completely degrade xylan to xylotriose | Ustilago maydis |