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Literature summary for 3.2.1.8 extracted from

  • McPhillips, K.; Waters, D.M.; Parlet, C.; Walsh, D.J.; Arendt, E.K.; Murray, P.G.
    Purification and characterisation of a beta-1,4-xylanase from Remersonia thermophila CBS 540.69 and its application in bread making (2014), Appl. Biochem. Biotechnol., 172, 1747-1762.
    View publication on PubMed
Search for more literature for 3.2.1.8

Application

Application Comment Organism
nutrition application of enzyme in bread making at low dosages of 0.297 International Xylanase Units/g shows its suitability to increase loaf volume by 8.0% compared with the control bread. Enzyme increases loaf softness by 19.6% while reducing bread staling by 20.4% up to 4 days of storage Remersonia thermophila

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 25% residual activity Remersonia thermophila
Cu2+ 1 mM, 2% residual activity Remersonia thermophila
Zn2+ 1 mM, 2.3% residual activity Remersonia thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 soluble oat spelt xylan Km value is 1.84 mg/ml, pH 6.0, 60°C Remersonia thermophila
additional information
-
 arabinoxylan Km value is 2.2 mg/ml, pH 6.0, 60°C Remersonia thermophila
additional information
-
 beechwood xylan Km value is 2.48 mg/ml, pH 6.0, 60°C Remersonia thermophila
additional information
-
 insoluble oat spelt xylan Km value is 7.01 mg/ml, pH 6.0, 60°C Remersonia thermophila

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Remersonia thermophila
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, 125% of initial activity Remersonia thermophila

Organism

Organism UniProt Comment Textmining
Remersonia thermophila
-
-
-
Remersonia thermophila CBS 540.69
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Remersonia thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.5
-
 pH 6.0, 60°C Remersonia thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinoxylan + H2O
-
 Remersonia thermophila ?
-
 ?
arabinoxylan + H2O
-
 Remersonia thermophila CBS 540.69 ?
-
 ?
beechwood xylan + H2O
-
 Remersonia thermophila ?
-
 ?
beechwood xylan + H2O
-
 Remersonia thermophila CBS 540.69 ?
-
 ?
insoluble oat spelt xylan + H2O best substrate Remersonia thermophila ?
-
 ?
insoluble oat spelt xylan + H2O best substrate Remersonia thermophila CBS 540.69 ?
-
 ?
additional information enzyme acts xylanolytic with no side-activities against other plant polysaccharides Remersonia thermophila ?
-
 ?
additional information enzyme acts xylanolytic with no side-activities against other plant polysaccharides Remersonia thermophila CBS 540.69 ?
-
 ?
soluble oat spelt xylan + H2O best substrate Remersonia thermophila ?
-
 ?
soluble oat spelt xylan + H2O best substrate Remersonia thermophila CBS 540.69 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Remersonia thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1034
-
 beechwood xylan pH 6.0, 60°C Remersonia thermophila
1492
-
 soluble oat spelt xylan pH 6.0, 60°C Remersonia thermophila
1657
-
 insoluble oat spelt xylan pH 6.0, 60°C Remersonia thermophila
1785
-
 arabinoxylan pH 6.0, 60°C Remersonia thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Remersonia thermophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 insoluble oat spelt xylan kcat/Km value is 236 mg per ml and s, pH 6.0, 60°C Remersonia thermophila
additional information
-
 beechwood xylan kcat/Km value is 417 mg per ml and s, pH 6.0, 60°C Remersonia thermophila
additional information
-
 arabinoxylan kcat/Km value is 809 mg per ml and s, pH 6.0, 60°C Remersonia thermophila
additional information
-
 soluble oat spelt xylan kcat/Km value is 810 mg per ml and s, pH 6.0, 60°C Remersonia thermophila