Cloned (Comment) | Organism |
---|---|
the chimeric enzymes, CorA-XynA and CorA-XynAG3, the parental enzyme XynA, and the thermostable variant XynAG3 are produced in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the fusion of the 1642-bp laccase (CorA) with either the 555-bp xylanase (XynA) or the thermostable variant (XynAG3) are performed by insertion of the xylanase into a surface loop of the laccase. The resulting chimeric constructs of 2197 bp contain a central region composed of the XynA or XynAG3 sequence flanked by the regions of the CorA encoding the N-terminal residues 1216 (forming the 5' region of the chimera) and the C-terminal region comprising residues 217513 of CorA. As a consequence, the final construct results in two linkage points between the laccase and xylanase domains | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
1,4-beta-D-xylan | Km for chimeric enzyme CorA-XynA at pH 6.5, 65°C: 6.3 mg/ml, KM for chimeric enzyme CorA-XynAG3 at pH 7.5, 75°C: 5.3 mg/ml, Km for xylanase XynA at pH 6.5, 55°C: 5.5 mg/ml, Km for thermostabel xylanase variant XynAG3 at pH 7.5, 70°C: 5.1 mg/ml | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
parental enzyme XynA, SDS-PAGE | Bacillus subtilis |
85500 | - |
chimeric enzymes CorA-XynA and CorA-XynAG3, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the chimeric enzymes, CorA-XynA and CorA-XynAG3, the parental enzyme XynA, and the thermostable variant XynAG3 are produced in Escherichia coli | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-beta-D-xylan + H2O | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-xylanase | - |
Bacillus subtilis |
XynA | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
xylanase XynA | Bacillus subtilis |
65 | - |
chimeric enzyme CorAXynA | Bacillus subtilis |
70 | - |
thermostable variant XynAG3 and chimeric enzyme CorAXynAG3 | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the thermostable variant XynAG3 loses 90% of its activity after 10 min, and after 30 min no activity is observed. The chimeric CotA-XynAG3 retains 60% activity after 10 min and about 20% activity after 120 min. The half-lives of the chimeric enzymes XynAG3 and CotA-XynAG3 are 2.2 and 21 min | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
61 | - |
1,4-beta-D-xylan | pH 7.5, 70°C, thermostable xylanase variant XynAG3, calculated as D-xylose liberated from 1,4-beta-D-xylan per second | Bacillus subtilis | |
65 | - |
1,4-beta-D-xylan | pH 7.5, 70°C, chimeric enzyme CorA-XynAG3, calculated as D-xylose liberated from 1,4-beta-D-xylan per second | Bacillus subtilis | |
75 | - |
1,4-beta-D-xylan | pH 6.5, 65°C, chimeric enzyme CorA-XynA, calculated as D-xylose liberated from 1,4-beta-D-xylan per second | Bacillus subtilis | |
82 | - |
1,4-beta-D-xylan | pH 6.5, 55°C, xylanase XynA, calculated as D-xylose liberated from 1,4-beta-D-xylan per second | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
xylanase XynA and chimeric enzyme CorA-XynA | Bacillus subtilis |
7.5 | - |
thermostabel xylanase variant XynAG3 and chimeric enzyme CorA-XynAG3 | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
1,4-beta-D-xylan | kcat/Km for chimeric enzyme CorA-XynA at pH 6.5, 65°C: 12 ml/mg*ml, kcat/KM for chimeric enzyme CorA-XynAG3 at pH 7.5, 75°C: 12 ml/mg*ml, kcat/Km for xylanase XynA at pH 6.5, 55°C: 14.9 ml/mg*ml, kcat/Km for thermostabel xylanase variant XynAG3 at pH 7.5, 70°C: 11.9 ml/mg*ml | Bacillus subtilis |