Cloned (Comment) | Organism |
---|---|
gene xynD, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain X33 | Talaromyces funiculosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Talaromyces funiculosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Talaromyces funiculosus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41540 | - |
x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation | Talaromyces funiculosus |
46000 | - |
x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation | Talaromyces funiculosus |
64000 | - |
x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation | Talaromyces funiculosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces funiculosus | Q5ZNB1 | gene xynD | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant protein is N- and O-glycosylated | Talaromyces funiculosus |
Purification (Comment) | Organism |
---|---|
recombinant XynD 1.9fold from Pichia pastoris strain X33 by ultrafiltration and gel filtration | Talaromyces funiculosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity with different arabinoxylans, overview | Talaromyces funiculosus |
3 | 3.9 | purified recombinant enzyme, pH 5.0, 70°C | Talaromyces funiculosus |
50.4 | - |
purified recombinant enzyme, substrate birchwood xylan, pH 5.0, 70°C | Talaromyces funiculosus |
115.9 | - |
purified recombinant enzyme, substrate high viscosity arabinoxylan, pH 5.0, 70°C | Talaromyces funiculosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinoxylan + H2O | low to high viscosity arabinoxylans, and water-extractable wheat arabinoxylans with different ratio arabinose:xylose, overview | Talaromyces funiculosus | ? | - |
? | |
beech wood xylan + H2O | - |
Talaromyces funiculosus | ? | - |
? | |
birch wood xylan + H2O | - |
Talaromyces funiculosus | ? | - |
? | |
additional information | the catalytic efficiency increases slightly with increasing their chain length with a small difference of the XynD catalytic efficiency against the different xylooligosaccharides | Talaromyces funiculosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation | Talaromyces funiculosus |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-xylanase | - |
Talaromyces funiculosus |
GH10 xylanase D | - |
Talaromyces funiculosus |
More | XynD belongs to the GH10 family | Talaromyces funiculosus |
XynD | - |
Talaromyces funiculosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Talaromyces funiculosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified recombinant enzyme, 180 min, no loss of activity | Talaromyces funiculosus |
80 | - |
purified recombinant enzyme, 10 min, loss of 80% activity | Talaromyces funiculosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Talaromyces funiculosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Talaromyces funiculosus | isoelectric focusing | - |
5.9 |
General Information | Comment | Organism |
---|---|---|
additional information | XynD possesses four catalytic subsites with a high energy of interaction with the substrate and a fifth subsite with a small energy of interaction | Talaromyces funiculosus |