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Literature summary for 3.2.1.8 extracted from
- Cloning, expression and characterization of glycoside hydrolase family 11 endoxylanase from Bacillus pumilus ARA (2011), Biotechnol. Lett., 33, 1407-1416.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xynA, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in of His-tagged XynA in Escherichia coli strain DH10B and JM109 | Bacillus pumilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the activity of the xylanase is not significantly affected by metal ions and EDTA | Bacillus pumilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 23000, SDS-PAGE | Bacillus pumilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | Q5EFR9 | gene XynA | - |
| Bacillus pumilus ARA | Q5EFR9 | gene XynA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged XynA from Escherichia coli by nickel affinity and anion exchange chromatography | Bacillus pumilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the main product of hydrolysis by XynA are xylobiose, xylotriose, xylotetraose and other xylooligosaccharides, only trace amount of xylose appear | Bacillus pumilus | ? | - |
? |  |
| additional information | the main product of hydrolysis by XynA are xylobiose, xylotriose, xylotetraose and other xylooligosaccharides, only trace amount of xylose appear | Bacillus pumilus ARA | ? | - |
? | |
| oat spelt xylan + H2O | - |
Bacillus pumilus | ? | - |
? | |
| oat spelt xylan + H2O | - |
Bacillus pumilus ARA | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23000, SDS-PAGE | Bacillus pumilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | a glycoside hydrolase family 11 endoxylanase | Bacillus pumilus |
| XynA | - |
Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant enzyme | Bacillus pumilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
purified recombinant enzyme, 2 h, stable | Bacillus pumilus |
| 60 | - |
purified recombinant enzyme, 1 h, over 50% activity remaining | Bacillus pumilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
recombinant enzyme | Bacillus pumilus |
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