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Literature summary for 3.2.1.8 extracted from
- Highly thermostable xylanase purified from Rhizomucor miehei NRL 3169 (2011), Acta Biol. Hung., 62, 85-94.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the enzyme is a potentially strong candidate for industrial and commercial application in pulp bleaching | Rhizomucor miehei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Rhizomucor miehei |  |
| DTT | - |
Rhizomucor miehei | |
| EDTA | - |
Rhizomucor miehei | |
| Fe3+ | - |
Rhizomucor miehei | |
| Hg2+ | - |
Rhizomucor miehei | |
| Mg2+ | - |
Rhizomucor miehei | |
| Mn2+ | - |
Rhizomucor miehei | |
| Zn2+ | - |
Rhizomucor miehei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Rhizomucor miehei | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Rhizomucor miehei | |
| Cu2+ | activates | Rhizomucor miehei | |
| K+ | activates | Rhizomucor miehei | |
| Na+ | activates | Rhizomucor miehei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, SDS-PAGE | Rhizomucor miehei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizomucor miehei | - |
- |
- |
| Rhizomucor miehei NRL 3169 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 29.1fold to homogeneity by ammonium sulfate fractionation, gel filtration and anion exchange chromatography | Rhizomucor miehei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1849 | - |
purified enzyme, substrate birch wood xylan, pH 5.0, 75°C | Rhizomucor miehei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| birch wood xylan + H2O | - |
Rhizomucor miehei | ? | - |
? | |
| birch wood xylan + H2O | - |
Rhizomucor miehei NRL 3169 | ? | - |
? | |
| additional information | no activity with cellulose, cellobiose, and carboxymethyl cellulose | Rhizomucor miehei | ? | - |
? | |
| additional information | no activity with cellulose, cellobiose, and carboxymethyl cellulose | Rhizomucor miehei NRL 3169 | ? | - |
? | |
| oat spelt xylan + H2O | - |
Rhizomucor miehei | ? | - |
? | |
| oat spelt xylan + H2O | - |
Rhizomucor miehei NRL 3169 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, SDS-PAGE | Rhizomucor miehei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoxylanase | - |
Rhizomucor miehei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Rhizomucor miehei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | activity range | Rhizomucor miehei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 75 | purified enzyme, 60 min, completely stable | Rhizomucor miehei |
| 80 | - |
purified enzyme, 60 min, 80% activity remaining | Rhizomucor miehei |
| 85 | - |
purified enzyme, 60 min, 40% activity remaining | Rhizomucor miehei |
| 90 | - |
purified enzyme, half-life is 30 min, 20% activity remaining after 60 min | Rhizomucor miehei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.5 | - |
Rhizomucor miehei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.5 | activity range | Rhizomucor miehei |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | endoxylanase is the key enzyme for xylan depolymerization, which attacks the main chain of xylan | Rhizomucor miehei |
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Release 2023.1 (January 2023)
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