Cloned (Comment) | Organism |
---|---|
gene xynA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
G23R | construction of a XynA mutant with increased pH stability, Computational design-based molecular engineering, overview | Bacillus subtilis |
Q175K | construction of a XynA mutant with increased pH stability, Computational design-based molecular engineering, overview | Bacillus subtilis |
T10H | construction of a XynA mutant with increased pH stability, Computational design-based molecular engineering, overview | Bacillus subtilis |
W9H | construction of a XynA mutant with increased pH stability, Computational design-based molecular engineering, overview | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Bacillus subtilis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P18429 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Bacillus subtilis | ? | - |
? | |
beta-1,4-xylan + H2O | from birchwood | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4-xylanase | - |
Bacillus subtilis |
endoxylanase | - |
Bacillus subtilis |
More | the enzyme belongs to the glycosyl hydrolase family 11, GH11 | Bacillus subtilis |
XynA | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | activity range, inactive at 60°C, temperature profile of recombinant His-tagged wild-type and mutant enzymes, overview | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
recombinant His-tagged wild-type enzyme | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | activity range, pH dependency of recombinant His-tagged wild-type and mutant enzymes, overview | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
molecular mechanisms governing the pH-dependent stability of GH11 proteins, overview | Bacillus subtilis |
3 | - |
30-70% remaining activity for the enzyme mutants, 23% for the wild-type | Bacillus subtilis |
4 | - |
75-95% remaining activity for the enzyme mutants, 57% for the wild-type | Bacillus subtilis |