Cloned (Comment) | Organism |
---|---|
gene Mxyn10, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Esccherichia coli strain BL21(DE3) | Demequina sp. JK4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for birchwood xylan is 3.45 mg/ml | Demequina sp. JK4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, about, sequence calculation | Demequina sp. JK4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Demequina sp. JK4 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Demequina sp. JK4 | B9VSZ3 | gene Mxyn10 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Esccherichia coli strain BL21(DE3) by nickel affinity chromatography | Demequina sp. JK4 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.7 | - |
purified recombinant enzyme, substrate oat spelt xylan | Demequina sp. JK4 |
8.9 | - |
purified recombinant enzyme, substrate birchwood xylan | Demequina sp. JK4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Demequina sp. JK4 | ? | - |
? | |
beta-1,4-xylan + H2O | from oalt spelt and birchwood | Demequina sp. JK4 | ? | - |
? | |
additional information | Mxyn10 shows activity on cellulose. Glu163 and Glu271 are the most likely residues located in the catalytic site of Mxyn10 | Demequina sp. JK4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, about, sequence calculation | Demequina sp. JK4 |
More | Mxyn10 contains a catalytic domain from positions 36 to 339 and a carbohydrate-binding module at the C-terminus. Glu163 and Glu271 are the most likely residues located in the catalytic site of Mxyn10 | Demequina sp. JK4 |
Synonyms | Comment | Organism |
---|---|---|
More | Mxyn10 contains a catalytic domain that belongs to the glycoside hydrolase family 10 and a carbohydrate-binding module belonging to family 2 | Demequina sp. JK4 |
Mxyn10 | - |
Demequina sp. JK4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Demequina sp. JK4 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 90 | enzyme assay range, 50% of maximal activity at 45°C and 60°C | Demequina sp. JK4 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | purified recombinant enzyme, stable | Demequina sp. JK4 |
60 | - |
purified recombinant enzyme, 80% activity remaining | Demequina sp. JK4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Demequina sp. JK4 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | enzyme assay range, over 50% of maximal activity at pH 5.0 and pH 7.5 | Demequina sp. JK4 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 10 | purified recombinant enzyme, stable | Demequina sp. JK4 |