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Literature summary for 3.2.1.8 extracted from

  • Meng, X.; Shao, Z.; Hong, Y.; Lin, L.; Li, C.; Liu, Z.
    A novel pH-stable, bifunctional xylanase isolated from a deep-sea microorganism, Demequina sp. JK4 (2009), J. Microbiol. Biotechnol., 19, 1077-1084.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Mxyn10, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Esccherichia coli strain BL21(DE3) Demequina sp. JK4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km for birchwood xylan is 3.45 mg/ml Demequina sp. JK4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
x * 49000, about, sequence calculation Demequina sp. JK4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Demequina sp. JK4
-
?
-
?

Organism

Organism UniProt Comment Textmining
Demequina sp. JK4 B9VSZ3 gene Mxyn10
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Esccherichia coli strain BL21(DE3) by nickel affinity chromatography Demequina sp. JK4

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7
-
purified recombinant enzyme, substrate oat spelt xylan Demequina sp. JK4
8.9
-
purified recombinant enzyme, substrate birchwood xylan Demequina sp. JK4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
Demequina sp. JK4 ?
-
?
beta-1,4-xylan + H2O from oalt spelt and birchwood Demequina sp. JK4 ?
-
?
additional information Mxyn10 shows activity on cellulose. Glu163 and Glu271 are the most likely residues located in the catalytic site of Mxyn10 Demequina sp. JK4 ?
-
?

Subunits

Subunits Comment Organism
? x * 49000, about, sequence calculation Demequina sp. JK4
More Mxyn10 contains a catalytic domain from positions 36 to 339 and a carbohydrate-binding module at the C-terminus. Glu163 and Glu271 are the most likely residues located in the catalytic site of Mxyn10 Demequina sp. JK4

Synonyms

Synonyms Comment Organism
More Mxyn10 contains a catalytic domain that belongs to the glycoside hydrolase family 10 and a carbohydrate-binding module belonging to family 2 Demequina sp. JK4
Mxyn10
-
Demequina sp. JK4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Demequina sp. JK4

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 90 enzyme assay range, 50% of maximal activity at 45°C and 60°C Demequina sp. JK4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 purified recombinant enzyme, stable Demequina sp. JK4
60
-
purified recombinant enzyme, 80% activity remaining Demequina sp. JK4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Demequina sp. JK4

pH Range

pH Minimum pH Maximum Comment Organism
3 11 enzyme assay range, over 50% of maximal activity at pH 5.0 and pH 7.5 Demequina sp. JK4

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 10 purified recombinant enzyme, stable Demequina sp. JK4