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Literature summary for 3.2.1.8 extracted from

  • Rasmussen, L.E.; Soerensen, J.F.; Meyer, A.S.
    Kinetics and substrate selectivity of a Triticum aestivum xylanase inhibitor (TAXI) resistant D11F/R122D variant of Bacillus subtilis XynA xylanase (2010), J. Biotechnol., 146, 207-214.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D11F/R122D the mutant shows highly decreased sensitivity to inhibitor Triticum aestivum xylanase inhibitor compared to wild-type enzyme Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Triticum aestivum xylanase inhibitor TAXI, 44-50% inhibition of the wild-type enzyme, nearly no inhibition of enzyme mutant D11F/R122D Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arabinoxylan + H2O Bacillus subtilis
-
xylo-oligosaccharide + ? product analysis, overview ?
beta-1,4-xylan + H2O Bacillus subtilis
-
xylo-oligosaccharide + ? product analysis, overview ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P18429
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinoxylan + H2O
-
Bacillus subtilis xylo-oligosaccharide + ? product analysis, overview ?
arabinoxylan + H2O higher activity with water-unextractable, than-extractable arabinoxylans, overview Bacillus subtilis xylo-oligosaccharide + ? product analysis, overview ?
beta-1,4-xylan + H2O
-
Bacillus subtilis xylo-oligosaccharide + ? product analysis, overview ?
beta-1,4-xylan + H2O from birchwood xylan and wheat bran Bacillus subtilis xylo-oligosaccharide + ? product analysis, overview ?

Subunits

Subunits Comment Organism
More structure homology modelling of wild-type and mutant enzymes, overview Bacillus subtilis

Synonyms

Synonyms Comment Organism
More the enzyme belongs to glycosyl hydrolase family 11, GH11 Bacillus subtilis
xylanase
-
Bacillus subtilis
XynA
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Bacillus subtilis