Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Paenibacillus sp. W-61 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a DELTAxyn5 mutant, as well as a xyn5DELTASLH mutant, lacking the SLH domain, grow poorly and produce minimal amounts of Xyn1 and Xyn3 on water-insoluble xylan. The xyn5DELTASLH mutant is secreted to the culture medium | Paenibacillus sp. W-61 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | Xyn5, binds to the cell surface via the SLH domain | Paenibacillus sp. W-61 | 9986 | - |
extracellular | - |
Paenibacillus sp. W-61 | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Paenibacillus sp. W-61 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. W-61 | - |
genes xyn1, xyn3, and xyn5 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Paenibacillus sp. W-61 | ? | - |
? | |
beta-1,4-xylan + H2O | water-soluble xylan | Paenibacillus sp. W-61 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | Xyn1, Xyn3, and Xyn5 are extracellular enzymes of the glycoside hydrolase families 11, 30, and 10, respectively | Paenibacillus sp. W-61 |
Xyn1 | - |
Paenibacillus sp. W-61 |
Xyn3 | - |
Paenibacillus sp. W-61 |
Xyn5 | - |
Paenibacillus sp. W-61 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Paenibacillus sp. W-61 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Paenibacillus sp. W-61 |
Organism | Comment | Expression |
---|---|---|
Paenibacillus sp. W-61 | xylobiose, generated from water-insoluble xylan decomposition by Xyn5, is the most active inducer for xyn1 and xyn3, while xylotriose is the best inducer for xyn5. Enhancement of Xyn5 expression precedes the induction of other xylanase genes by water-insoluble xylan | up |
General Information | Comment | Organism |
---|---|---|
physiological function | two roles of Xyn5, localized on the cell surface, in water-insoluble xylan utilization: generation of the xylo-oligosaccharide inducers of all the xyn genes from water-insoluble xylan and attachment of the cells to the substrate so that the generated inducers can be immediately taken up by cells to activate expression of the xyn system, overview. Cell surface-bound Xyn5 is crucial for growth of strain W-61 in the water-insoluble xylan medium | Paenibacillus sp. W-61 |