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Literature summary for 3.2.1.8 extracted from

  • Fukuda, M.; Watanabe, S.; Yoshida, S.; Itoh, H.; Itoh, Y.; Kamio, Y.; Kaneko, J.
    Cell surface xylanases of the glycoside hydrolase family 10 are essential for xylan utilization by Paenibacillus sp. W-61 as generators of xylo-oligosaccharide inducers for the xylanase genes (2010), J. Bacteriol., 192, 2210-2219.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Paenibacillus sp. W-61

Protein Variants

Protein Variants Comment Organism
additional information a DELTAxyn5 mutant, as well as a xyn5DELTASLH mutant, lacking the SLH domain, grow poorly and produce minimal amounts of Xyn1 and Xyn3 on water-insoluble xylan. The xyn5DELTASLH mutant is secreted to the culture medium Paenibacillus sp. W-61

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface Xyn5, binds to the cell surface via the SLH domain Paenibacillus sp. W-61 9986
-
extracellular
-
Paenibacillus sp. W-61
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Paenibacillus sp. W-61
-
?
-
?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. W-61
-
genes xyn1, xyn3, and xyn5
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
Paenibacillus sp. W-61 ?
-
?
beta-1,4-xylan + H2O water-soluble xylan Paenibacillus sp. W-61 ?
-
?

Synonyms

Synonyms Comment Organism
More Xyn1, Xyn3, and Xyn5 are extracellular enzymes of the glycoside hydrolase families 11, 30, and 10, respectively Paenibacillus sp. W-61
Xyn1
-
Paenibacillus sp. W-61
Xyn3
-
Paenibacillus sp. W-61
Xyn5
-
Paenibacillus sp. W-61

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Paenibacillus sp. W-61

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Paenibacillus sp. W-61

Expression

Organism Comment Expression
Paenibacillus sp. W-61 xylobiose, generated from water-insoluble xylan decomposition by Xyn5, is the most active inducer for xyn1 and xyn3, while xylotriose is the best inducer for xyn5. Enhancement of Xyn5 expression precedes the induction of other xylanase genes by water-insoluble xylan up

General Information

General Information Comment Organism
physiological function two roles of Xyn5, localized on the cell surface, in water-insoluble xylan utilization: generation of the xylo-oligosaccharide inducers of all the xyn genes from water-insoluble xylan and attachment of the cells to the substrate so that the generated inducers can be immediately taken up by cells to activate expression of the xyn system, overview. Cell surface-bound Xyn5 is crucial for growth of strain W-61 in the water-insoluble xylan medium Paenibacillus sp. W-61