Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.8 extracted from

  • Yin, L.J.; Lin, H.H.; Chiang, Y.I.; Jiang, S.T.
    Bioproperties and purification of xylanase from Bacillus sp. YJ6 (2010), J. Agric. Food Chem., 58, 557-562.
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol native enzyme Bacillus sp. (in: Bacteria)
glutathione native enzyme Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ inhibits the native enzyme Bacillus sp. (in: Bacteria)
Fe3+ inhibits the native enzyme Bacillus sp. (in: Bacteria)
Hg2+ inhibits the native enzyme Bacillus sp. (in: Bacteria)
leupeptin inhibits the native enzyme Bacillus sp. (in: Bacteria)
N-ethylmaleimide inhibits the native enzyme Bacillus sp. (in: Bacteria)
N-tosyl-L-phenylalanine chloromethyl ketone inhibits the native enzyme Bacillus sp. (in: Bacteria)
PMSF inhibits the native enzyme Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates the native enzyme Bacillus sp. (in: Bacteria)
K+ activates the native enzyme Bacillus sp. (in: Bacteria)
Mg2+ activates the native enzyme Bacillus sp. (in: Bacteria)
Na+ activates the native enzyme Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 x * 19000, native enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
beta-1,4-xylan + H2O Bacillus sp. (in: Bacteria) YJ6
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) YJ6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 678.1fold from strain YJ6 to homogeneity by ammonium sulfate fractionation, dialysis, cation exchange chromatography, ultrafiltration, and gel filtration Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1436
-
 purified native enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
beta-1,4-xylan + H2O from oat spelt, birchwood, or beechwood Bacillus sp. (in: Bacteria) ?
-
 ?
beta-1,4-xylan + H2O
-
 Bacillus sp. (in: Bacteria) YJ6 ?
-
 ?
beta-1,4-xylan + H2O from oat spelt, birchwood, or beechwood Bacillus sp. (in: Bacteria) YJ6 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 19000, native enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
xylanase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified native enzyme, stable Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 purified native enzyme, stable Bacillus sp. (in: Bacteria)