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Literature summary for 3.2.1.8 extracted from
- Thermostability and xylan-hydrolyzing property of endoxylanase expressed in yeast Saccharomyces cerevisiae (2009), Biotechnol. Bioprocess Eng., 14, 639-644.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xynB, expression in Saccharomyces cerevisiae strain SEY2102, the recombinant enzyme is secreted, and the extracellular endoxylanase expressed in yeast shows an enhanced thermal stability due to the N-linked glycosylation | Bacillus subtilis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | Q45VU2 | xynB; gene xynB | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the extracellular endoxylanase expressed in yeast shows an enhanced thermal stability due to the N-linked glycosylation, the native enzyme in Bacillus subtilis is not glycosylated | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,4-xylan + H2O | from birchwood | Bacillus subtilis | xylobiose + xylotriose + ? | major products with equimolar ratio | ? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,4-endoxylanase | - |
Bacillus subtilis |
| endoxylanase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
glycosylated recombinant enzyme, 30 h, 50% remaining activity. Unglycosylated native enzyme, 12 h, 50% activity remaining | Bacillus subtilis |
| 70 | - |
glycosylated recombinant enzyme, 1 h, 90% remaining activity | Bacillus subtilis |
| 80 | - |
glycosylated recombinant enzyme, 1 h, 86% remaining activity | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Bacillus subtilis |
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Release 2023.1 (January 2023)
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