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Literature summary for 3.2.1.8 extracted from
- Improvement of Aspergillus sulphureus endo-beta-1,4-xylanase expression in Pichia pastoris by codon optimization and analysis of the enzymic characterization (2010), Appl. Biochem. Biotechnol., 160, 1321-1331.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xynB, constitutive expression of wild-type enzyme and enzyme without signal sequence in Pichia pastoris strain X-33, the recombinant enzyme is secreted to the culture medium, nmethod optimization, overview | Aspergillus sulphureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Aspergillus sulphureus |  |
| Mn2+ | - |
Aspergillus sulphureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Aspergillus sulphureus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no or poor inhibition by EDTA, Na+, K+, Ca2+, Mg2+, and Fe2+, overview | Aspergillus sulphureus | |
| Zn2+ | activates | Aspergillus sulphureus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,4-xylan + H2O | Aspergillus sulphureus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus sulphureus | Q3S401 | gene xynB | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,4-xylan + H2O | - |
Aspergillus sulphureus | ? | - |
? | |
| beta-1,4-xylan + H2O | oat spelt xylan, degradation into xylo-oligosaccharides of various lengths | Aspergillus sulphureus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,4-xylanase | - |
Aspergillus sulphureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant enzyme | Aspergillus sulphureus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | 50% of maximal activity at 20°C, 20% at 70°C | Aspergillus sulphureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified recombinant enzyme, half-life is 30 min | Aspergillus sulphureus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6551 | - |
beta-1,4-xylan | recombinant wild-type enzyme, pH 5.0, 50°C, oat spelt xylan | Aspergillus sulphureus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
recombinant enzyme | Aspergillus sulphureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.4 | 7.4 | recombinant enzyme, rapid decrease in activity above pH 6.4, profile, overview | Aspergillus sulphureus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
purified recombinant enzyme, over 90% activity remaining after 2 h | Aspergillus sulphureus |
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