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Literature summary for 3.2.1.8 extracted from
- Structure-based mutagenesis of Penicillium griseofulvum xylanase using computational design (2008), Proteins, 72, 1298-1307.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and mutants introduced into the pGEX-1kT/PgXynA plasmid and expressed in Escherichia coli BL21 (pLys) strain | Penicillium griseofulvum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTAD130 | retains specific activity comparable to the wild-type | Penicillium griseofulvum |
| F14Y | retains specific activity comparable to the wild-type | Penicillium griseofulvum |
| Q121R | shows a marked 33% increase in specific activity, mainly due to a 2fold increase in kcat. It does not alter the hydrolysis product profile of wheat arabinoxylan, shows highest increase in activity on low substituted xylan | Penicillium griseofulvum |
| R7T | retains specific activity comparable to the wild-type | Penicillium griseofulvum |
| S129G | sensitivity to the xylanase inhibitor protein, XIP-I | Penicillium griseofulvum |
| S129G/DELTAD130 | lowest Ki compared to tested natural enzymes | Penicillium griseofulvum |
| S129G/S44D | low specific activity | Penicillium griseofulvum |
| S129G/S44N | sensitivity to the xylanase inhibitor protein, XIP-I | Penicillium griseofulvum |
| S44A | loses both pH-dependence profile and activity, reduces activity mainly due to a reduction in kcat whereas the apparent affinity remains unchanged | Penicillium griseofulvum |
| S44D | shows only slight alteration in Km and Vmax, reduces activity mainly due to a reduction in kcat whereas the apparent affinity remains unchanged. It shifts the activity to acidic pHs by ca. 1 unit, decreasing the optimum pH to 4.5. It has a broader pH profile retaining ca. 60% of its maximum activity at pH 3.0 as compared to the wild-type | Penicillium griseofulvum |
| S44N | shows only slight alteration in Km and Vmax, reduces activity mainly due to a reduction in kcat whereas the apparent affinity remains unchanged. It shifts the activity to alkaline pHs by ca. 0.5 unit with a pH optimum of 5.5 | Penicillium griseofulvum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| XIP-I | xylanase inhibitor protein | Penicillium griseofulvum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium griseofulvum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type and mutants | Penicillium griseofulvum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 45 | - |
mutant S44A, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 95 | - |
mutant S129G/S44D, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 493 | - |
mutant S44D, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 627 | - |
mutant S129G/DELTAD130, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 764 | - |
mutant S129G, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 866 | - |
mutant S129G/S44N, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 954 | - |
mutant S44N, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 1085 | - |
mutant R7T, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 1100 | - |
wild-type, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 1146 | - |
mutant DELTAD130, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 1226 | - |
mutant F14Y, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
| 1464 | - |
mutant Q121R, at 30°C, with wheat arabinoxylan as substrate | Penicillium griseofulvum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| wheat arabinoxylan + H2O | - |
Penicillium griseofulvum | ? | - |
? |  |
| xylohexaose + H2O | - |
Penicillium griseofulvum | D-xylose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GH11 xylanase | - |
Penicillium griseofulvum |
| xylanase | - |
Penicillium griseofulvum |
| XynA | - |
Penicillium griseofulvum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27 | - |
wheat arabinoxylan | mutant S44A, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum | |
| 298 | - |
wheat arabinoxylan | mutant S44D, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum | |
| 448 | - |
wheat arabinoxylan | mutant R7T, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum | |
| 454 | - |
wheat arabinoxylan | mutant S44N, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum | |
| 457 | - |
wheat arabinoxylan | mutant S129G/DELTAD130, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum | |
| 484 | - |
wheat arabinoxylan | mutant S129G, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum | |
| 534 | - |
wheat arabinoxylan | mutant S129G/S44N, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum | |
| 545 | - |
wheat arabinoxylan | wild-type, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum | |
| 601 | - |
wheat arabinoxylan | mutant DELTAD130, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum | |
| 773 | - |
wheat arabinoxylan | mutant F14Y, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum | |
| 1063 | - |
wheat arabinoxylan | mutant Q121R, calculated as xylose units released from wheat arabinoxylan | Penicillium griseofulvum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Penicillium griseofulvum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | displays over 50% activity, around 30% of activity still at pH 7.3 | Penicillium griseofulvum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | retains full activity for 2 h at 30°C | Penicillium griseofulvum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000039 | - |
XIP-I | mutant S129G/DELTAD130 | Penicillium griseofulvum | |
| 0.000082 | - |
XIP-I | mutant S129G | Penicillium griseofulvum | |
| 0.00009 | - |
XIP-I | mutant S129G/S44N | Penicillium griseofulvum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Penicillium griseofulvum | - |
- |
8.6 |
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